Confocal Microscopy Facility

共焦显微镜设备

• 批准号: BB/F011458/1
• 负责人: James Haseloff
• 金额: $ 28.73万
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2009
• 资助国家: 英国
• 起止时间: 2009 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FF011458%2F1
• 关键词: Confocal; Microscopy; Facility; Confocal; Microscopy; Facility

The development of the first practical confocal laser scanning microscope in the 1980's was a major advance in optical microscopy. These microscopes allow researchers to obtain optical sections from intact specimens up to a depth of several hundred microns, and are now widely used in plant biology for the visualisation of biological processes and ultrastructure in intact and living plant tissues. A successful JREI application in 1998 led to the installation of an early generation confocal microscope in the Department of Plant Sciences in the University of Cambridge. As a result, plant researchers here have contributed heavily to the early development and application of confocal imaging techniques. Our existing facilities need upgrading in order to maintain our lead in the development of new fluorescent proteins, transgenic marker lines in Arabidopsis, algal and crop systems, new microtechniques for 3D imaging, software for image segmentation, visualisation and quantitation, and the application of advanced optical techniques to the analysis of cell, protein and metabolite dynamics in a wide variety of algal and plant systems.

1980年代首次实用共聚焦激光扫描显微镜的发展是光学显微镜的重大进步。这些显微镜使研究人员可以从完整的标本中获得至少数百微米深度的光学切片，现在被广泛用于植物生物学中，用于可视化生物过程和完整植物组织中的生物学过程和超微结构。 1998年成功的JREI应用程序导致在剑桥大学植物科学系安装了早期共聚焦显微镜。结果，这里的植物研究人员为共聚焦成像技术的早期开发和应用做出了巨大贡献。我们现有的设施需要升级，以维持我们在新的荧光蛋白的开发中保持领先地位，拟南芥中的转基因标记线，藻类和作物系统，新的用于3D成像的微技术，用于图像分割，可视化和定量的软件，以及用于分析细胞，蛋白质和植物系统的分析，用于分析元素和化学的动力学。