MULTIPLE SCLEROSIS: A PROGRAM PROJECT

多发性硬化症：一个计划项目

• 批准号: 3100144
• 负责人: BARRY G ARNASON
• 金额: $ 94.52万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-04-01 至 1991-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3100144
• 关键词: multiple sclerosis

Project 1 is directed towards a systematic study of suppressor cell function in multiple sclerosis. Changes in surface phenotype will be analyzed in conjunction with functional assays looking for liaison or lack of liaison between the two. Effects of activating and abrogating agents will also be determined. Project 2 examines oligodendrocyte plasma membrane and myelin compartments and oligodendrocyte-neuron interactions as well as the effects of proteoglycans and glycoproteins secreted by oligodendrocytes on these interactions. Project 3 examines a unique surface glycoprotein (gp 105) of myelin and oligodendrocytes. Its structure will be determined as will its distribution and hopefully its function. Monoclonal antibodies to gp105 will be raised and cDNA's coding for it obtained. Project 4 is directed towards an antigen-specific immunoglobulin response within the brain using the TMEV virus-mediated demyelinating disease model that he has been studying. He will also develop TMEV-reactive T cell lines and clones and determine the viral epitopes against which they react as well as whether they will react with CNS antigens. Project 5 addresses the potential role of neuroleukin production by astrocytes in immunocyte activation in brain. Project 6 employs patch clamp techniques to study ion channels on oligodendrocytes and on T suppressor cell lines and clones derived from multiple sclerosis patients and controls.

项目1针对抑制细胞的系统研究 多发性硬化症的功能。 表面表型的变化将 与寻求的功能测定法一起分析 两者之间的联络或缺乏联络。 激活的影响 也将确定废除剂。 项目2 检查少突胶质细胞质膜和髓鞘 车厢和少突胶质细胞 - 神经元相互作用以及 蛋白聚糖和糖蛋白分泌的作用 这些相互作用的少突胶质细胞。 项目3检查 髓磷脂的独特表面糖蛋白（GP 105）和 少突胶质细胞。它的结构将与其结构一样 分发并希望其功能。 单克隆抗体 GP105将升高，并为其进行编码。 项目4 针对抗原特异性免疫球蛋白反应 使用TMEV病毒介导的脱髓鞘的大脑内 他一直在研究的疾病模型。 他也会发展 TMEV反应性T细胞系和克隆，并确定病毒 他们反应的表位以及是否会 与CNS抗原反应。 项目5解决了潜在角色 星形胶质细胞在免疫细胞激活中产生神经素素 在大脑中。 项目6采用斑块夹技术来研究离子 少突胶质细胞和T抑制细胞系上的通道以及 克隆来自多个硬化症患者和对照组。