INVESTIGATION OF A TESTIS PARACRINE FACTOR, P-MOD-S

睾丸旁分泌因子 P-MOD-S 的研究

• 批准号: 3087059
• 负责人: JOHN N. NORTON
• 金额: $ 5.31万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-04-01 至 1992-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3087059
• 关键词: RNA; Sertoli cells; biological signal transduction; cell cell interaction; cyclic AMP; electrophoresis; gene expression; hormone regulation /control mechanism; ion exchange chromatography; laboratory rat; nucleic acid hybridization; nucleic acid probes; spermatogenesis; testis; transferrin

Testicular somatic cells plays an integral role in the maintenance and control of spermatogenesis. Analysis of somatic cell-cell interactions within the testis has demonstrated that testicular peritubular cells secrete P-Mod-S, a locally derived, nonmitogenic paracrine factor. P-Mod-S the functions of Sertoli cells maintains germinal cell development. P-Mod-S has been shown to stimulate Sertoli cells to a greater degree than any single regulatory agent known to influence the cell. Therefore, peritubular cell-Sertoli cell interactions, mediated via P-Mod-S, is postulated to play a critical role in the maintenance and control of testicular function. The goals of this research proposal are:1) determine the mechanism of action of P-Mod-S on the Sertoli cell;2) determine the effects of P-Mod-S on the expression of Sertoli cell genes important for testicular function; and 3) identify and initially characterize the P-Mod-S receptor. The completion of these goals will provide additional understanding of the paracrine factor P-Mod-S and its significance in the maintenance and control of spermatogenesis. Thus, further investigation of the testicular paracrine factor P-Mod-S, will provide information needed for the potential future design of agents to act on the testis as a contraceptive or for the prevention of infertility.

睾丸体细胞在维护中起着不可或缺的作用 控制精子发生。 分析体细胞 - 细胞相互作用 在睾丸中表明睾丸周围细胞 分泌P-Mod-S，一种局部衍生的非遗传性旁分泌因子。 P-Mod-s Sertoli细胞的功能维持生发细胞的发育。 P-Mod-s 已证明可以比任何人都更大程度地刺激Sertoli细胞 已知会影响细胞的单一调节剂。 所以， 通过p-mod-s介导的周围细胞静脉细胞相互作用， 假定在维护和控制中发挥关键作用 睾丸功能。 该研究建议的目标是：1）确定 P-Mod-S对Sertoli细胞的作用机理； 2）确定 P-Mod-S对Sertoli细胞基因表达的影响 睾丸功能； 3）识别并最初表征P-Mod-S 受体。 这些目标的完成将提供更多 了解旁分泌因子p-mod-s及其在 维持和控制精子发生。 因此，进一步研究 睾丸旁分泌因子P-Mod-S将提供所需的信息 为了使代理商的未来设计在睾丸上作用 避孕或预防不育。