FETAL SWALLOWING--CENTRAL DIPSOGENIC MECHANISMS

胎儿吞咽——中枢促生机制

• 批准号: 6024379
• 负责人: Michael Glenn Ross
• 金额: $ 6.57万
• 依托单位: LUNDQUIST INSTITUTE FOR BIOMEDICAL INNOVATION AT HARBOR-UCLA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6024379
• 关键词: angiotensin II; arginine vasopressin; atrial natriuretic peptide; body fluid osmolarity; body water; brain regulatory center; cerebral cortex; embryo /fetus; homeostasis; immunocytochemistry; microinjections; physiology; receptor binding; sheep; stereotaxic techniques; swallowing

DESCRIPTION (Adapted from the Investigator's Abstract): The proposed studies will explore the ontogeny of fetal central osmotic-dipsogenic mechanisms to develop an understanding of normal and abnormal fluid homeostatic responses. The investigators hypothesize: a) osmotic-dipsogenic responsiveness develops as a result of maturation of neural pathways integrating the subfornical organ (sSFO) and organum vasculosum of the lamina terminalis (OVLT) with the median preoptic nucleus (MnPO). b) The development of endocrine modulation of dipsogenic responses coincides with the onset of specific receptor binding in the SFO, OVLT, and MnPO. And c) Fetal dipsogenic responses to hyperosmolarity may be fundamentally different from the adult, as a result of the developmental pattern of dipsogenic mechanisms. A stepwise series of experiments is proposed: 1) The ontogeny of the responsiveness of putative osmotic dipsogenic central nuclei (SFO, OVLT, MnPO) will be examined with neuronal activation (cFos) staining and nuclei miroinjections; 2) The essential role of these nuclei will be investigated via nuclei microablation; 3) The development of interconnecting dipsogenic neuronal pathways will be investigated with neural tract tracing techniques; and 4) The maturation of the endocrine modulation of dipsogenic responses will be correlated with changes in cerebral endocrine receptor binding and immunochemical staining.

描述（改编自调查员的摘要）：提议 研究将探讨胎儿中央渗透降解的个体发育 对正常和异常流体的理解的机制 稳态反应。 调查人员假设：a） 由于成熟而发展 整合次型器官（SSFO）和细胞器的神经通路 薄片末端（OVLT）​​的血管中位核中位核 （MNPO）。 b）倾向反应的内分泌调节的发展 与SFO，OVLT和 mnpo。 c）胎儿对高渗性的反应可能是 由于发展而与成年人根本不同 倾向机制的模式。 逐步的一系列实验是 提议：1）推定渗透的反应性的个体发育 将使用神经元检查次基因中心核（SFO，OVLT，MNPO） 激活（CFO）染色和核注射核； 2）重要角色 这些核将通过核微观研究研究； 3） 相互连接的倾向神经元途径的发展将是 通过神经道追踪技术进行了研究； 4）成熟 倾向反应的内分泌调节将与 脑内分泌受体结合和免疫化学染色的变化。