AQUAPORIN GENE EXPRESSION IN HUMAN FETAL MEMBRANE

人胎膜中水通道蛋白基因的表达

• 批准号: 7606210
• 负责人: Michael Glenn Ross
• 金额: $ 0.13万
• 依托单位: LUNDQUIST INSTITUTE FOR BIOMEDICAL INNOVATION AT HARBOR-UCLA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-02-01 至 2007-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7606210
• 关键词: Amniotic Fluid; Cell membrane; Cesarean section; Chorion; Computer Retrieval of Information on Scientific Projects Database; Fetal Membranes; Fetal Movement; Freezing; Funding; Gene Expression; Grant; Growth and Development function; Homeostasis; Human; Institution; Life; Liquid substance; Mediating; Membrane; Molecular; Nitrogen; Oligohydramnios; Pathway interactions; Placenta; Play; Pregnancy; Purpose; RNA; Reagent; Regulation; Research; Research Personnel; Resources; Reverse Transcriptase Polymerase Chain Reaction; Role; Source; Technology; Tissues; Umbilical cord structure; United States National Institutes of Health; Water; absorption; amnion; fetal; trizol; water channel; water flow

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Amniotic fluid (AF) is an essential accompaniment of normal pregnancy, necessary for fetal movement, growth and development. Oligohydramnios (OH), or reduced AF volume, occurs in 8 to 38% of all pregnancies. The intramembranous pathway of AF fluid absorption (across the amniotic membrane) has recently been recognized as a critical regulatory path for AF resorption, contributing importantly to AF volume homeostasis. Yet the underlying molecular and cellular mechanisms for water absorption across the amniotic membranes remain unknown. Since the discovery of AQP water channels about a decade ago, AQPs are increasingly recognized to play an important role in water transport across cell membranes. IM resorption of AF (water flow across the amnion and perhaps chorion, to the fetal vasculature) is potentially mediated via AQP water channel(s). However, of the eleven identified mammalian AQPs, few have been examined in fetal membranes. We have demonstrated AQP8 expression in both human and ovine amnion, chorion and placenta. These results suggest that AQP8, or other water channels may provide a pathway for fluid transport across fetal membranes, contributing to AF volume homeostasis. The purpose of the project is to investigate the aquaporins gene expression in human fetal membrane to further determine which water channels contribute to the regulation of amniotic fluid resorption. Human placenta and fetal membranes from normal term pregnancy will be studied. Immediately upon cesarean section delivery, amnion, chorion, placenta, and umbilical cord will be dissected and frozen in liquid nitrogen. Tissues will be stored at -70C until further analysis. Total RNA will be isolated from these tissues using Trizol reagent (Life Technologies, Gaithersburg, MD) for RT-PCR. RT-PCR will be used to detect the aquaporins gene expression in these tissues. We will use the discarded human placenta and fetal membranes from the subject upon delivery. Nothing will be done to the subjects.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 羊水（AF）是正常妊娠的重要伴奏，是胎儿运动，生长和发育所必需的。在所有妊娠的8％至38％中，少聚糖（OH）（OH）或AF量减小。 AF液吸收的膜内途径（跨羊膜）最近被认为是AF吸收的关键调节途径，对AF体积体积稳态有重要贡献。然而，跨羊膜吸水的潜在分子和细胞机制尚不清楚。 由于大约十年前发现了AQP水通道，因此越来越多地认识到AQP在整个细胞膜的水运输中发挥重要作用。 IM吸收AF的吸收（遍布羊膜或绒毛膜的水流，胎儿脉管系统）可能通过AQP水通道介导。但是，在11个鉴定出的哺乳动物AQP中，很少有人在胎儿膜中进行检查。我们已经证明了人类和卵子羊膜，绒毛膜和胎盘的AQP8表达。这些结果表明，AQP8或其他水通道可能为跨胎儿膜的流体转运提供途径，从而导致AF量体内稳态。该项目的目的是研究人类胎儿膜中的水通道蛋白基因表达，以进一步确定哪些水通道有助于调节羊水吸收。将研究正常妊娠的人胎盘和胎儿膜。立即在剖腹产分段后，羊膜，绒毛膜，胎盘和脐带进行剖析，并在液氮中冷冻。组织将存储在-70C之前，直到进一步分析。将使用Trizol试剂（Life Technologies，Gaithersburg，MD）将总RNA与RT-PCR分离。 RT-PCR将用于检测这些组织中的水通道蛋白基因表达。 分娩后，我们将使用该受试者中丢弃的人胎盘和胎儿膜。对象什么都不会做。