HETEROGENEITY IN THE VOMERONASAL SYSTEM

犁鼻系统的异质性

• 批准号: 2900049
• 负责人: MIMI N. HALPERN
• 金额: $ 12.06万
• 依托单位: SUNY DOWNSTATE MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2900049
• 关键词: G protein; Golgi apparatus; autoradiography; biological polymorphism; cell age; cell population study; chemoreceptors; dendrites; electrophysiology; epithelium; genetic polymorphism; immunocytochemistry; light microscopy; neuroanatomy; olfactory lobe; opossums; vomeronasal systems

The main olfactory and vomeronasal (VN) systems are the major nasal chemosensory systems of most terrestrial vertebrates. A major question that has eluded solution for both systems is the mechanism of stimulus coding. One of the prominent current hypotheses proposes that sensory receptor cells with different complements of receptor proteins on their distal processes send their axons to segregated portions of the olfactory bulbs. In the past several years our laboratory has demonstrated that the accessory olfactory bulb (AOB) of the grey, short-tailed opossum, Monodelphis domestica is heterogeneous. A number of "markers" are expressed in or strain more strongly the rostral AOB than the caudal AOB. These "markers" include olfactory marker protein (OMP), the lactin Vicia villosa (VVA), NADPH-diphorase, and Gi2a. In addition, we have observed that in the AOB Goa is located predominantly in its posterior subdivision. This heterogeneity in the chemoarchitecture of the AOB may reflect a fundamental organizational dichotomy within the vomeronasal system that corresponds to a functional dichotomy and it is the goal of the studies described herein to pursue this problem. This goal will be accomplished by 1. determining whether the vomeronasal epithelium contains separate population of receptor cells t hat are distinguishable with the "markers" that differentially stain the A OB; 2. determining whether the receptor cells that project to the rostral AOB; 3. determining whether two populations of receptor cells, already discriminated with antibodies to the two G proteins, are of different ages; 4. determining whether athe mitral/tufted cells of one subdivision of the AOB have principal dendrite restricted to that subdivision; and 5. determining whether athe centripetal projections of the anterior AOB differ from those of the posterior AOB.

主嗅觉和呕吐（VN）系统是主要的鼻 大多数陆地脊椎动物的化学体积系统。 一个主要问题 这两个系统的解决方案是刺激的机制 编码。 当前突出的假设之一提出了感觉 受体蛋白质上不同补体的受体细胞在其上 远端过程将其轴突发送到嗅觉的隔离部分 灯泡。 在过去的几年中，我们的实验室表明 灰色，短尾负鼠的配件嗅球（AOB）， 单层家族是异质的。 许多“标记”是 比尾aoB更强烈地表达或强烈应变。 这些“标记”包括嗅觉标记蛋白（OMP），乳蛋白vicia Villosa（VVA），Nadph-Diphorase和Gi2a。 另外，我们已经观察到 在AOB果阿中，主要位于其后部分区。 AOB化学结构中的这种异质性可能反映了A 沃马纳纳纳斯系统内的基本组织二分法 对应于功能性二分法，这是研究的目标 本文描述了解决这个问题。 这个目标将通过 1。确定呕吐性上皮是否包含单独的 受体细胞的种群可以用“标记”来区分 差异弄脏了a ob； 2。确定受体是否 投射到主aob的细胞； 3。确定是否两个 受体细胞的种群，已经用抗体歧视 两种G蛋白不同年龄。 4。确定是否ATHE AOB一个细分的二尖瓣/簇状细胞具有主树突 仅限于该细分； 5。确定是否a 前AOB的centripetal投影与 后AOB。