CRYSTAL STRUCT OF DOUBLE SEX PROTEIN & SEX DETERMINING REGION OF Y CHROMOSOME

双性蛋白的晶体结构

• 批准号: 6315709
• 负责人: ZHULI WAN
• 金额: $ 0.42万
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-15 至 2000-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6315709
• 关键词: biomedical resource; structural biology

The human sex-determining gene on the Y chromosome, termed SRY, has been isolated by oppositional cloning, compelling evidence now exists equating SRY with the testis-determining factor. The sry gene product is an HMG box protein whose DNA binding activity is vital for testis formation as sex-reversed patients with SRY mutations lack this activity in vitro. The in viva DNA target for SRY, however, remains elusive. It is now 75 years since insulin was first used in treatment of diabetes. Since then, many of the biophysical properties of the hormone have been characterized. In order to study the relationship of structure and function, we have got some high active insulin mutants. We have obtained crystals of short human SRY and insulin mutants. Good energy resolution is obtained from monochromator. The data were collected in flash frozen. The data were collected in flash frozen, using monochromator on the beamline 14 BM-D CCD detector.

Y染色体上的人类性别确定基因，称为Sry， 现在通过对立克隆，令人信服的证据隔离了 存在等于睾丸确定的因素。 Sry基因 产品是HMG盒蛋白，其DNA结合活性对于 睾丸会形成，因为性转向的SRY突变患者缺乏此事 体外活性。 然而，viva dna靶标仍然存在 难以捉摸。 自胰岛素首次用于治疗以来已经75年了 糖尿病。 从那以后，许多生物物理特性 激素已被表征。 为了研究这种关系 结构和功能，我们有一些高活性胰岛素 突变体。 我们已经获得了短的人类和胰岛素的晶体 突变体。 良好的能量分辨率是从单色器获得的。 这 数据收集在Flash冷冻中。 数据收集到Flash 冻结，使用光束线14 BM-D CCD检测器上的单色器。