SINGLE-CHAIN INSULIN AND MUTANTS STUDIES

单链胰岛素和突变体研究

• 批准号: 7956834
• 负责人: ZHULI WAN
• 金额: $ 0.94万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7956834
• 关键词: Biological; C-Peptide; Computer Retrieval of Information on Scientific Projects Database; Data; Diabetic Neuropathies; Endotoxemia; Funding; Grant; Home environment; In Vitro; Inflammation; Institution; Insulin; Kidney Diseases; Nuclear; PPAR gamma; Peptides; Play; Reporting; Research; Research Personnel; Resolution; Resources; Role; Source; Structure; United States National Institutes of Health; Work; improved; mutant; research study; structural biology; synchrotron radiation; transcription factor; vascular inflammation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. To continue our proposal work, the proposal number: GUP-8345 We propose to collect high resolution native and mutants data of the single-chain insulin. Home-source experiments (Raxix IV) diffract to 2.5 A resolution. We feel that the x-ray source at BioCARS will provide for better diffraction from small crystal. Insulin connecting peptide (C-peptide) aids the folding of single-chain insulin has been considered to have little biological activity. C-peptide has been shown to improve diabetic neuropathy and nephropathy as well as vascular inflammation. In vitro studies have reported that c-peptide may activate peroxisome proliferator-activated receptor-gamma, a nuclear transcription factor that plays a regulatory role in inflammation. this study was desiged to investigate the biological effects of c-peptide during endotoxemia. We expect to collect more set of diffraction data of single-chain insulin and mutants using synchrotron radiation and solve its crystal structure and study on the relationship od structure and function.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 为了继续我们的提案工作，提案编号：GUP-8345 我们建议收集单链胰岛素的高分辨率天然和突变体数据。家庭源实验（Raxix IV）衍射为2.5分辨率。我们认为，生物级的X射线源将提供从小晶体中更好的衍射。胰岛素连接肽（C肽）有助于单链胰岛素的折叠，被认为具有很少的生物学活性。已显示C肽可改善糖尿病神经病和肾病以及血管炎症。体外研究报告说，C肽可能会激活过氧化物酶体增殖物激活的受体伽马，这是一种在炎症中起调节作用的核转录因子。这项研究被认为是研究内毒素血症中C肽的生物学作用。我们希望使用同步加速器辐射收集更多单链胰岛素和突变体的衍射数据，并解决其晶体结构并研究关系OD结构和功能。