HOMEODOMAIN PROTEIN AND CELL TYPE SPECIFICITY

同源域蛋白和细胞类型特异性

• 批准号: 2707731
• 负责人: MELODY V SIEGLER
• 金额: $ 18.41万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-09-28 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2707731
• 关键词: Drosophilidae; cell type; developmental neurobiology; gene expression; homeobox genes; invertebrate embryology

The present work is part of a general effort to determine the role of homeobox genes in the development and maintenance of neuronal specificity in the central nervous system. A growing number of studies have demonstrated specific defects in brain and spinal cord, and in a number of non-neuronal tissues, when homeobox genes have been disrupted during development. Although the CNS defects resulting from homeobox disruption are typically described in terms of particular anatomical structures or brain regions, the defects must ultimately reside in alterations of the phenotype of individual neurons, and further, result from the altered expression of particular structural genes. Homeobox genes are commonly regarded as developmental genes. Nonetheless, some continue expression into adulthood, and it is therefore speculated that they have a role in maintaining the integrity of neuronal structure, connectivity, or function. We use invertebrate models to test the effects of different patterns of homeodomain gene expression during defined times of embryogenesis and monitor the changes with a battery of cell-specific markers. We wish to determine whether cell types are altered, and whether there are other effects on formation of neuronal circuits.

目前的工作是确定作用的一般努力的一部分 同源基因在神经元的开发和维持中 中枢神经系统的特异性。 越来越多的研究 已经证明了大脑和脊髓中的特定缺陷，以及 当同源性基因被破坏时，非神经组织的数量 在开发过程中。 虽然中枢神经系统的缺陷是由同源的 通常用特定解剖学来描述中断 结构或大脑区域，缺陷必须最终居住在 单个神经元的表型的改变，进一步的结果 来自特定结构基因的表达改变。 同型 基因通常被视为发育基因。 尽管如此，有些人 继续表达成年，因此据推测 他们在维持神经元结构的完整性中起作用， 连接或功能。 我们使用无脊椎动物模型来测试 同源域基因表达不同模式的影响 定义的胚胎发生时间并用电池监测变化 细胞特异性标记。我们希望确定单元类型是否 改变，以及是否对神经元的形成还有其他影响 电路。