LINEAL ORIGIN OF NEURON GROUPS

神经元群的线性起源

• 批准号: 3075172
• 负责人: MELODY V SIEGLER
• 金额: $ 6.7万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-01 至 1995-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3075172
• 关键词: Orthoptera; bromodeoxyuridine; cell differentiation; central nervous system disorders; developmental neurobiology; efferent nerve; electrophysiology; embryo /fetus; gamma aminobutyrate; ganglions; gene expression; glutamine; immunocytochemistry; interneurons; motor neurons; neuroanatomy; neurochemistry; neurons; neurophysiology; neurotransmitters

The candidate will pursue a research career that focuses on the embryonic differentiation of clonally-related neurons and on the way that differences in neuronal morphology, physiology and biochemistry (transmitter type) lead to differing anatomical and physiological connections in the neuronal circuitry of the mature central nervous system. Experiments proposed will address the lineal origins of stereotyped neuron groups which we have recently described in the adult ganglia of the grasshopper Schistocerca americana, and the expression of differing phenotypic traits in individually-identified neurons in these groups. A major prerequisite for the realization of these goals is for the candidate to develop proficiency in a number of methodologies, primarily those related to lineage analysis in the embryo. Expertise in these methods can be learned partially at Emory University and partially at the institutions of colleagues. A Research Career Development Award would greatly facilitate this process by providing the candidate with greater opportunity for research activities (85%) through reduction in commitments to teaching and university service. Our specific aims involve the study of one group of neurons that we suggest arise embryonically from a single neuroblast. The group contains some 20 neurons of diverse phenotype, including glutaminergic motor neurons, intersegmental interneurons that may be GABA-ergic, a multibranced efferent that may be homologous to peptidergic neurons from other insects and an indeterminate number of local interneurons. We will describe the anatomical and electrophysiological properties of the neurons in the group in the adult, so that we can identify each as an individual. We will determine which properties are shared by all members of the group and which are unique to some. We will then begin work on the embryo, to test our hypothesis that the neurons are clonally related. To trace lineage we will use conventional techniques for the intracellular injection of dye and newly-developed techniques for labeling with an altered nucleoside BUdR. The labeling with dyes, BUdR and other antibodies will be done in a closely timed series to determine the relative time of origin of the various neurons and of acquisition of differing phenotypic traits.

候选人将从事专注于胚胎的研究职业 克隆相关神经元的区分以及差异的方式 在神经元形态，生理和生物化学（发射器类型）中 神经元中的解剖学和生理联系不同 成熟中枢神经系统的电路。提出的实验意愿 解决我们拥有的刻板神经元组的线性起源 最近在Grasshopper Schistocerca的成年神经节中描述了 美国，以及不同表型特征的表达 这些组中的单独识别神经元。一个主要的先决条件 这些目标的实现是使候选人提高水平 在许多方法论中，主要是与谱系分析有关的方法 在胚胎中。这些方法的专业知识可以在Emory中部分学习 大学，部分是同事机构。研究 职业发展奖将通过提供极大地促进这一过程 有更多机会进行研究活动的候选人（85％） 通过减少对教学和大学服务的承诺。我们的 具体目的涉及我们建议的一组神经元的研究 由单个神经细胞胚胎出现。该小组包含大约20个 各种表型的神经元，包括谷氨酰胺能运动神经元， 可能是gaba-ergic的段性中间神经元，一种多纤维传出 这可能与其他昆虫的肽能神经元同源 不确定的局部神经元数。我们将描述解剖学 和组中神经元的电生理特性 成人，以便我们可以识别每个人。我们将确定 小组的所有成员共享哪些属性，哪些是 某些人是独一无二的。然后，我们将开始在胚胎上工作，以测试我们的 假设神经元是克隆相关的。为了追踪血统我们将 使用常规技术进行细胞内注射染料和 用改变的核苷芽标记的新发达的技术。 用染料，BUDR和其他抗体的标签将在一个紧密的 定时系列以确定各种原点的相对时间 神经元和获得不同表型特征的获取。