SIGNAL TRANSDUCTION DURING SWARMER CELL DIFFERENTIATION

集群细胞分化过程中的信号转导

• 批准号: 2597869
• 负责人: Rasika M Harshey
• 金额: $ 18.88万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2597869
• 关键词: Escherichia coli; Salmonella typhimurium; bacterial genetics; bacterial proteins; biological signal transduction; cell differentiation; chemotaxis; cilium /flagellum motility; gene expression; gene mutation; life cycle; microorganism growth

DESCRIPTION: The long term goal of this research is to provide a genetic framework for understanding the physiological responses of bacteria growing in surface biofilms. These responses have a large impact on our ecology and our health. Our model system is the surface-induced swarmer cell differentiation response discovered in our laboratory in E. coli and S. typhimurium, and known to be elicited by several flagellated bacteria. Swarmer cells are generally long and multinucleate, always hyperflagellated, and can move rapidly over the agar surface in a coordinated manner. There is evidence that the differentiated swarmer-cell stage of some bacteria facilitates pathogenic associations with host tissue. Almost nothing is known about the molecular signaling mechanism of surface sensing. In organisms in which swarming motility has been studied in some detail, the chemotaxis system has been shown to play an important role. The specific aims of this proposal are to understand the mechanism by which the chemotaxis components participate in surface signal transduction, which eventually turns on swarmer cell-specific gene expression. We believe that understanding the genetic basis of swarming in such well-characterized bacteria as E. coli and S. typhimurium will provide valuable clues to understanding surface-specific behaviors in other microorganisms.

描述：这项研究的长期目标是提供遗传 了解细菌生长的生理反应的框架 在表面生物膜中。 这些回应对我们的生态和 我们的健康。 我们的模型系统是表面引起的蜂群 在我们的大肠杆菌和S.的实验室中发现的分化反应 鼠伤寒，已知被几种鞭毛细菌引起。 堆积细胞通常长且多核细胞，始终多粉状， 并可以以协调的方式快速在琼脂表面上移动。 那里 证据表明某些细菌的分化蜂窝阶段 促进与宿主组织的致病性关联。 几乎没有 知道表面传感的分子信号传导机制。 在 详细研究了蜂群运动的生物， 趋化系统已显示出重要作用。 具体 该提议的目的是了解 趋化成分参与表面信号转导，这 最终打开蜂单细胞特异性基因表达。 我们相信 了解在这种良好的特征中蜂群的遗传基础 细菌作为大肠杆菌和鼠伤寒S.将为 了解其他微生物的表面特异性行为。