The flagellar motor as a sensor

作为传感器的鞭毛马达

• 批准号: 8916804
• 负责人: Rasika M Harshey
• 金额: $ 27.86万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-01 至 2016-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8916804
• 关键词: Bacteria; Bacterial Infections; Behavior; Binding; Binding Sites; Cells; Chemicals; Chemotaxis; Complex; Environment; Escherichia coli; Experimental Designs; Film; Flagella; Health; Knowledge; Lead; Light; Liquid substance; Locales; Microbial Biofilms; Modeling; Molecular; Molecular Conformation; Monitor; Motor; Organelles; Output; Pathogenesis; Pathway interactions; Play; Power Sources; Property; Proteins; Protons; Recruitment Activity; Role; Rotation; Running; Salmonella; Sensory; Signal Transduction; Signaling Molecule; Solid; Speed; Surface; Swimming; Testing; Transcriptional Activation; Variant; base; cell motility; insight; microbial community; migration; receptor; research study; response; sensor; Bacteria; Bacterial Infections; Behavior; Binding; Binding Sites; Cells; Chemicals; Chemotaxis; Complex; Environment; Escherichia coli; Experimental Designs; Film; Flagella; Health; Knowledge; Lead; Light; Liquid substance; Locales; Microbial Biofilms; Modeling; Molecular; Molecular Conformation; Monitor; Motor; Organelles; Output; Pathogenesis; Pathway interactions; Play; Power Sources; Property; Proteins; Protons; Recruitment Activity; Role; Rotation; Running; Salmonella; Sensory; Signal Transduction; Signaling Molecule; Solid; Speed; Surface; Swimming; Testing; Transcriptional Activation; Variant; base; cell motility; insight; microbial community; migration; receptor; research study; response; sensor

DESCRIPTION (provided by applicant): The Flagellar Motor as a Sensor Bacteria use rotation of helical flagella to propel themselves either through bulk liquid (swimming), or through a thin film of liquid on a solid surface (swarming). Chemosensory pathways normally communicate environmental information to the bidirectional rotary motor, modulating its CW/CCW bias to optimize bacterial migration towards favorable locales. These pathways are well-understood. In E. coli and other bacteria, the motor is also itself a sensory organelle, allowing bacteria to adapt to chemical signals, respond directly to increased c-di- GMP, and mechanically 'sense' a solid surface. These pathways, particularly mechanosensing, are not understood. In light of recent evidence showing that the motor restructures its stator and rotor components during both chemosensing and mechanosensing, we propose a new testable model for how the motor acts as a mechanosensor. The model identifies the rotor-stator interface as the sensory hub of the motor. We consider a finite number of structural components that could receive and transduce sensory signals from this hub. These are: MotA/B, FliG, FliM, FliL, YcgR and H-NS. The first two aims of this proposal investigate remodeling of a subset of these components under situations where the motor elicits a distinct response to two internal signals - CheY~P and c-di-GMP. The third aim benefits from this knowledge to monitor remodeling of these components in response to mechano-signals. The insights derived from these studies are expected to eventually trace the input signal(s) at the motor to output responses that promote either motility or sessility (biofilms), both of which play critical roles i bacterial infection, surface colonization, pathogenesis, and persistence.

描述（由申请人提供）：鞭毛电动机作为传感器细菌使用螺旋鞭毛的旋转来推动自己通过散装液体（游泳）或通过 固体表面上的液体薄膜（蜂群）。化学感觉途径通常会将环境信息传达给双向旋转电动机，从而调节其CW/CCW偏置，以优化细菌迁移到有利的地区。这些途径被很好地理解。在大肠杆菌和其他细菌中，该电动机本身也是一个感觉细胞器，使细菌可以适应化学信号，直接响应增加的c-di-gmp，并机械地“感觉”固体表面。这些途径，尤其是机械感应，尚不清楚。鉴于最近的证据表明，电动机在化学传感和机械传感过程中都对其定子和转子组件进行了重组，我们提出了一个新的可测试模型，以实现电动机作为机械传感器的作用。该模型将转子定子界面识别为电动机的感觉中心。我们考虑有限数量的结构组件，这些组件可能会从该中心接收并传递感官信号。这些是：Mota/b，Flig，Flim，Flil，YCGR和H-NS。该提案的前两个目的研究了在电动机引起对两个内部信号的明显响应-CHEY〜P和C-DI-GMP的明显响应的情况下的这些组件子集的重塑。第三个目标受益于这些知识，可以监测这些组件对机械信号的重塑。预计从这些研究中得出的见解最终将追踪电机处的输入信号，以促进运动性或稳定性（生物膜）的输出响应，这两种响应都起着关键作用I细菌感染，表面定植，发病机理和持久性。