FETAL VELOCIMETRY AND AMINO ACID TRANSPORT IN PREGNANCY

妊娠期的胎速测定和氨基酸转运

• 批准号: 2674041
• 负责人: FREDERICK C BATTAGLIA
• 金额: $ 14.44万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-20 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2674041
• 关键词: acid base balance; aminoacid analyzer; aminoacid metabolism; aminoacid transport; blood chemistry; blood glucose; clinical research; gas chromatography mass spectrometry; high performance liquid chromatography; human pregnant subject; placental transfer; pregnancy; pregnancy circulation disorder; prenatal growth disorder; respiratory oxygenation; stable isotope diagnosis; statistics /biometry; ultrasound blood flow measurement; umbilical cord

The specific aims of this project involve clinical studies in normal pregnancies and pregnancies complicated by fetal growth restriction. The studies cover two aspects: 1) improvements in the classification of clinical severity using new fetal surveillance techniques and 2) studies of amino acid transport and metabolism using stable isotope methodology. The role of direct measurements of umbilical venous blood flow compared to arterial velocimetry measurements and fetal blood chemistry is examined around the framework of the classification of clinical severity. Similarly, the additional information acquired from single fetal organ velocimetry studies of the ductus venosus, fetal hepatic veins, fetal femoral artery, fetal middle cerebral artery are also compared to fetal umbilical arterial velocimetry and fetal chemistries. The studies of in vivo amino acid placental transport and metabolism is a follow up on our observations that fetal amino acid concentrations are significantly reduced in fetal growth restricted pregnancies. A non steady state study utilizing stable isoptomers of essential and non essential amino acids in pregnant women scheduled for cordocentesis examines in vivo transport for different groups of amino acids: a) to define differences in their relative rates of transport in vivo and b) to test whether amino acid transport is reduced in FGR pregnancies compared to normal pregnancies for all amino acids or a specific subset. Steady state stable isotope methodology is also used to test whether the fetal/maternal enrichment ratios for stable isoptomers of amino acids infused in the maternal circulation is lower in FGR pregnancies reflecting decreased transport and increased protein turnover. The relationship of maternal concentration to umbilical uptake for normal and FGR pregnancies is also investigated utilizing data on umbilical arteriovenous concentration differences at the time of cesarean section. All metabolic data are compared to the classification of clinical severity in FGR pregnancies using criteria we have previously published.

该项目的具体目的涉及正常的临床研究 怀孕和怀孕因胎儿生长限制而复杂。 研究涵盖了两个方面：1）改进的分类 使用新的胎儿监视技术和2）研究 使用稳定的同位素方法论的氨基酸转运和代谢。 脐静脉血流直接测量的作用 进行动脉速度测量和胎儿血液化学是 围绕临床严重程度分类的框架进行了检查。 同样，从单个胎儿器官获取的其他信息 导管，胎儿肝静脉，胎儿的速度学研究 还将股动脉，胎儿中大脑动脉与胎儿进行比较 脐动脉速度法和胎儿化学。 研究 体内氨基酸胎盘运输和代谢是我们的后续 观察到胎儿氨基酸浓度显着 减少胎儿生长受限怀孕。 非稳定状态 利用基本和非必需氨基的稳定的等异构体的研究 安排宿齿色穿刺术的孕妇中的酸检查体内 用于不同氨基酸组的运输：a）定义差异 它们在体内的相对运输速率和b）测试是否氨基 与正常相比，FGR妊娠的酸转运减少 所有氨基酸或特定子集的怀孕。 稳定稳定 同位素方法还用于测试胎儿/母体是否 注入在 FGR怀孕的孕产妇循环较低，反映出降低 运输并增加蛋白质更新。 孕产妇的关系 正常和FGR怀孕的脐带摄取也集中在 利用脐动静脉浓度的数据进行了研究 剖宫产时期的差异。 所有代谢数据都是 与FGR怀孕中临床严重程度的分类相比 使用以前发表的标准。