DETERMINATION OF RATES OF IN-VIVO PLACENTAL TRANSPORT OF 9 ESSENTIAL AMINO ACIDS

9 种必需氨基酸体内胎盘转运速率的测定

基本信息

批准号：
7605064
负责人：
FREDERICK C BATTAGLIA
金额：
$ 1.64万
依托单位：
UNIVERSITY OF COLORADO DENVER
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-03-01 至 2008-02-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7605064
关键词：
Amino Acid Transport System A Amino Acids Arteriogram Barium Belief Blood Vessels Blood specimen Cesarean section Characteristics Clinical Computer Retrieval of Information on Scientific Projects Database Computer software Defect Doppler Echocardiography Employee Strikes Essential Amino Acids Evaluation Failure Fetal Growth Retardation Funding Gelatin Goals Grant Growth Human Image In Vitro Institution Laboratories Morbidity - disease rate Organ Pathologic Patient currently pregnant Pattern Perinatal Placenta Pregnancy Preparation Rate Research Research Personnel Resources Scanning Electron Microscopy Sheep Source Techniques Time Tissues United States National Institutes of Health fetal blood in utero in vivo indexing mortality tool

Amino Acid Transport System A Amino Acids Arteriogram Barium Belief Blood Vessels Blood specimen Cesarean section Characteristics Clinical Computer Retrieval of Information on Scientific Projects Database Computer software Defect Doppler Echocardiography Employee Strikes Essential Amino Acids Evaluation Failure Fetal Growth Retardation Funding Gelatin Goals Grant Growth Human Image In Vitro Institution Laboratories Morbidity - disease rate Organ Pathologic Patient currently pregnant Pattern Perinatal Placenta Pregnancy Preparation Rate Research Research Personnel Resources Scanning Electron Microscopy Sheep Source Techniques Time Tissues United States National Institutes of Health fetal blood in utero in vivo indexing mortality tool

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项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Intrauterine growth restriction represents one of the most important causes of perinatal morbidity and mortality. In recent years, there has been an increased focus on the specific defect of a reduction in amino acid transport across the placenta in IUGR pregnancies. A reduction in placental transport of amino acids has been shown in growth retarded human pregnancies, both at the time of in utero fetal blood sampling and at Cesarean section. These findings are supported by in vitro studies of placentas from pregnancies complicated by IUGR. A specific reduction in these pregnancies has been shown for amino acids transported by System A (1). While there have been numerous studies of amino acid transport in perfused human placentas (2-4) and in our microvesicle preparations of the placenta (5,6), in vivo transport characteristics have not been described. In vivo studies carried out in pregnant sheep recently in our laboratory have shown striking differences in the rates of in vivo transport among the nine essential amino acids (15). The goal of the present study is to analyze the in vivo transport for all essential amino acids. It is our belief that these studies should identify an essential amino acid that would be a sensitive index of placental failure and in the long term may provide an additional very useful guide in the management of IUGR pregnancies. Human pregnancies complicated by IUGR have been shown to have abnormal placental vasculature [17-23] including abnormal branching patterns. Human placental vascular casts have been imaged using scanning electron microscopy (SEM) in both normal and pathologic placentas. Characteristic vascular changes have been observed, but there is little clinical correlation with doppler studies and clinical terms are often inconsistent or confusing. The placenta is a heterogeneous organ and SEM of vascular casts may be representative of focal changes only. As a second goal of this study we wish to evaluate the use of two techniques, barium-gelatin arteriograms (24) and serial tissue sections that have been reconstructed using 3-dimesional imaging software (25), to see if these could be useful tools in the evaluation of the placental vasculature in human pregnancies complicated by IUGR.

该副本是利用众多研究子项目之一由NIH/NCRR资助的中心赠款提供的资源。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是对于中心，这不一定是调查员的机构。宫内生长限制是围产期发病率和死亡率最重要的原因之一。近年来，在IUGR妊娠中，氨基酸转运的降低的特定缺陷越来越重视。在子宫胎儿血液采样和剖宫产时，氨基酸的胎盘运输的减少已经显示出智障的人类妊娠。这些发现得到了IUGR复杂妊娠的胎盘的体外研究。这些妊娠的特定降低已显示针对系统A（1）运输的氨基酸。尽管在灌注的人胎盘中进行了许多研究（2-4），以及我们的胎盘的微囊泡制剂（5,6），但尚未描述体内转运特性。最近在我们的实验室中，在怀孕绵羊中进行的体内研究表明，在九种必需氨基酸的体内运输速率上存在明显的差异（15）。本研究的目的是分析所有必需氨基酸的体内运输。我们认为，这些研究应确定必需的氨基酸，该氨基酸将是胎盘衰竭的敏感指数，从长远来看，可能会为IUGR妊娠管理提供其他非常有用的指南。 IUGR复杂的人类妊娠已被证明具有异常的胎盘脉管系统[17-23]，包括异常分支模式。在正常和病理胎盘中使用扫描电子显微镜（SEM）对人胎盘血管铸造进行了成像。已经观察到了特征性的血管变化，但是与多普勒研究几乎没有临床相关性，临床术语通常不一致或令人困惑。胎盘是一种异质器官，血管铸造的SEM可能仅代表焦点变化。作为这项研究的第二个目标，我们希望评估使用两种技术，钡明星蛋白动脉图（24）和已使用3段时间成像软件（25）重建的串行组织切片（25），以查看这些对人类妊娠复杂胎盘性血管造成的工具是否有用。