RADIATION INDUCED CHANGES IN TASTE

辐射引起的味觉变化

• 批准号: 2458563
• 负责人: MIRIAM R LINSCHOTEN
• 金额: $ 5.29万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2458563
• 关键词: biopsy; cell differentiation; citrates; clinical research; head /neck neoplasm; histochemistry /cytochemistry; human subject; ionizing radiation; keratin; neoplasm /cancer radiation therapy; quinine; radiation sensitivity; radiation therapy dosage; sodium chloride; squamous cell carcinoma; sucrose; taste; taste buds; taste disorders; taste threshold

Dysgeusia, the proposed studies will correlate psychophysical and histological changes in gustation during and following irradiation of the oral cavity. Since the detailed psychophysical testing will involve the anterior part of the tongue, histological analysis will focus on fungiform papillae. The experiments will compare the number of stainable taste pores observed in vivo to the psychophysical data. Biopsies of fungiform papillae will permit immunochemical analysis of taste buds. Established immunochemical markers of taste bud differentiation, i.e. gustducin and cytokeratin-19, will be used. These techniques will be applied to patients receiving irradiation to the oropharyngeal region as therapy for squamous cell carcinoma of the head and neck and to age-matched controls. Biopsy of taste papillae from the patients will be timed to coincide with the decline and recovery of gustatory sensitivity during and after radiation treatment. Subjects identified in the Ear, Nose and Throat Department at University Hospital will be tested for gustatory function prior to, during, and after radiation treatment. Detection thresholds will be measured globally (whole mouth) and locally (dorsal anterior surface of the tongue). Each subject will be tested with two of four tastants: sodium chloride, sucrose, citric acid and quinine sulfate. In this manner we will determine whether radiation produces similar degrees of sensitivity loss in all taste qualities and in response to similar radiation doses. Histochemical evaluation of taste buds will allow us to correlate the degree of taste loss with histological and cytochemical changes in the taste buds from the tested area of the tongue.

味觉障碍，拟议的研究将把心理物理和 辐射期间和之后味觉的组织学变化 口腔。由于详细的心理物理测试将涉及 舌头前部，组织学分析将重点关注 菌状乳头。实验将比较可染色的数量 味觉孔在体内观察到的心理物理数据。活组织检查 菌状乳头将允许对味蕾进行免疫化学分析。 建立味蕾分化的免疫化学标记物，即 将使用 gustducin 和 cytokeratin-19。这些技术将 适用于接受口咽区域照射的患者 头颈部鳞状细胞癌的治疗 年龄匹配的控制。将对患者的味觉乳头进行活检 时间与味觉敏感性的下降和恢复一致 放射治疗期间和之后。 大学耳鼻喉科确定的受试者 医院将在术前、术中和术后进行味觉功能测试。 放射治疗后。将在全球范围内测量检测阈值 （整个口腔）和局部（舌背前表面）。每个 受试者将用四种促味剂中的两种进行测试：氯化钠， 蔗糖、柠檬酸和硫酸奎宁。通过这种方式我们将 确定辐射是否会产生类似程度的灵敏度损失 在所有口味品质和对类似辐射剂量的反应中。 味蕾的组织化学评估将使我们能够将 味觉丧失的程度与组织学和细胞化学变化有关 来自舌头测试区域的味蕾。