PSYCHOPHYSICAL, STRUCTURAL AND FUNCTIONAL CORRELATES OF CHEMOSENSORY FUNCTION

化学感觉功能的心理物理、结构和功能相关性

• 批准号: 6442495
• 负责人: MIRIAM R LINSCHOTEN
• 金额: $ 24.33万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6442495
• 关键词: behavioral /social science research tag; clinical research; drug adverse effect; gamma radiation; human subject; neoplasm /cancer radiation therapy; neuropathology; olfactory disorder; radiation sensitivity; respiratory disorder chemotherapy; rhinitis; squamous cell carcinoma; steroids; taste buds; taste disorders; vomeronasal systems

This project focuses on disease states affect chemosensory epithelia. We turn our attention herein to two diseases-on involving taste, the other smell-possibly attributable to disturbances in the sensory epithelium: 1) radiation induced dysgeusia and 2) steroid dependent anosmia. We will utilize contemporary psychophysical, anatomical and physiological tests to examine the function and structure of the epithelium in control subjects and in the appropriate patient populations. This multidisciplinary approach will allow us to provide a more complete description of the functional and structural changes that correlate with sensory disturbances. Subjects diagnosed with squamous cell carcinoma of the head or neck will be tested for gustatory function prior to, during and after radiation treatment. Quantitative and qualitative taste changes will be tracked throughout the time period of loss and recovery of function. Vital staining of the tongue will allow us to measure taste pore density. Biopsies of fungiform papillae will permit us to measure taste bud integrity and function by means of the markers gustducin and cytokeratin- 19. In order to allow comparison between psychophysical and anatomical observations, all measures will be performed on the same area of the anterior dorsal surface of the tongue. Likewise, we will test subjects with allergic rhinitis for olfactory function prior to, during and after topical steroid treatment. Both detection ability and supra-threshold intensity perception will be determined unilaterally. Two biopsies of the olfactory epithelium will be analyzed using three histochemical markers: olfactory marker protein (OMP) and GAP43 as measures of cell maturity, and glucocorticoid receptor (GR) to determine changes in GR distribution. Parallel changes in membrane current and intracellular calcium induced by stimulation with odors will be measured in isolated olfactory receptor neurons using loose patch recording by stimulation with odors will be measured in isolated olfactory receptor neurons using loose patch recording with simultaneous calcium imaging. In addition, in order to assess possible changes in mucus ion homeostasis in this disease state, we will determine mucus ion content by flame photometry and ratio-imaging of ion-specific dyes.

该项目的重点是疾病状态影响化学感觉上皮。我们 将我们的注意力转向两种涉及口味的疾病，另一种疾病 气味可能归因于感觉上皮中的干扰：1） 辐射诱导的增生症和2）类固醇依赖性厌氧。我们将 利用当代心理物理，解剖学和生理测试 检查上皮在对照受试者中的功能和结构 并在适当的患者人群中。这个多学科 方法将使我们能够提供更完整的描述 与感觉相关的功能和结构变化 干扰。 被诊断为头部或颈部鳞状细胞癌的受试者将 在辐射之前，之中和之后测试 治疗。定量和定性品味的变化将被跟踪 在整个损失和恢复功能期间。必不可少的 舌头的染色将使我们能够测量口味孔密度。 真菌乳头的活检将使我们能够测量味蕾 通过标记剂和细胞角蛋白 - 完整性和功能 19。为了允许心理物理和解剖学之间的比较 观察结果，所有措施将在同一区域进行 舌头的前侧表面。 同样，我们将测试患有嗅觉过敏性鼻炎的受试者 在局部类固醇治疗之前，之中和之后的功能。两个都 检测能力和上阈值强度感知将是 单方面确定。嗅觉上皮的两个活检将是 使用三个组织化学标记分析：嗅觉标记蛋白（OMP） 和GAP43作为细胞成熟度和​​糖皮质激素受体（GR）的度量 确定GR分布的变化。膜的平行变化 通过气味刺激引起的电流和细胞内钙将 使用松散的斑块在孤立的嗅觉受体神经元中测量 通过用气味刺激记录将以孤立的嗅觉测量 与同时钙的片段记录的受体神经元 成像。另外，为了评估粘液离子的可能变化 在这种疾病状态下的稳态，我们将通过 离子特异性染料的火焰光度法和比率成像。