ARROW, A NEW WINGLESS SIGNALING COMPONENT

ARROW，一种新型无翼信号组件

• 批准号: 2673395
• 负责人: Kim A Caldwell
• 金额: $ 1.87万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2673395
• 关键词: Drosophilidae; developmental genetics; invertebrate embryology; molecular cloning

The long-term objectives of this work are to elucidate the molecular basis of pattern formation during development in Drosophila melanogaster. The characterization of a genetic component of the Wingless signaling pathway in the Drosophila embryonic epidermis will serve to define elements required for regulation of developmental decisions in invertebrates and, by analogy, vertebrates. The molecular cloning of arrow, a newly identified member of the Wingless pathway, will be performed and will facilitate subsequent studies designed to further identify the role arrow plays in the Wingless pathway. The generation of antibodies specific to the Arrow protein will aid in determining its cellular localization. Genetic epistasis and embryonic microinjection will provide information regarding the location, and possibly the mechanism of action, of arrow in the Wingless pathway. The implications of this work lie in the possibility that this genetic cascade will provide insights into mechanisms of vertebrate development as the Wingless pathway utilizes a novel form of signal transduction and is highly conserved evolutionarily.

这项工作的长期目标是阐明分子 在果蝇果蝇中发育过程中形成模式的基础。 无机信号的遗传成分的表征 果蝇胚胎表皮中的途径将用于定义 监管发展决策所需的要素 无脊椎动物，并以类比为脊椎动物。分子克隆 Arrow是无翼路径的新确定成员，将是 进行并将促进随后旨在进一步的研究 确定箭头在无翼途径中扮演的角色。一代 特定于箭头蛋白的抗体将有助于确定其 细胞定位。遗传学和胚胎显微注射 将提供有关位置的信息，甚至可能 动作机理，无翼路径中的箭头。含义 这项工作在于这种遗传级联的可能性 提供有关脊椎动物发育机制的见解 无翼途径利用一种新型的信号转导形式，IS 高度保守的进化。