MEASUREMENT OF ALBUMIN SYNTHETIC RATE DURING RECOVERY FROM INTENSE EXERCISE

剧烈运动恢复期间白蛋白合成率的测量

• 批准号: 6247116
• 负责人: ETHAN R NADEL
• 金额: $ 2.68万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-01 至 1997-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6247116
• 关键词: albumins; analytical method; exercise; human subject; protein biosynthesis; stable isotope

We measured hepatic albumin synthesis during the recovery from an intense intermittent exercise protocol previously shown to expand plasma volume. Using a primed-constant infusion of a stable isotopic tracer of phenylalanine, hepatic fractional synthetic rate (FSR) and absolute synthetic rate (ASR) of albumin were determined from the enrichment of phenylalanine in albumin and in the free intravascular precursor pool. Albumin FSR and ASR were 6.39+0.48% day-1and 120 +9mg (kgBW)-1 day-1, respectively, at 3 to 6 hours recovery from exercise; the FSR and ASR on the time control study day were 5.94+0.47% day-1 and 104+9mg (kgBW) -1 day-1 respectively. The 6% and 16% increases (P<0.05) in FSR and ASR following exercise were associated with an elevated plasma albumin content at 5 and 6 hours recovery (P<0.05), an increased total protein content throughout recovery (P<0.05), and a negative free water clearance (P<0.05) at 2,3 and 6.5 hours of recovery when compared to baseline values; these variables were unchanged from their baselines on the time control study day. Increased albumin content and reduced free water clearance both contribute to a retention of fluid within the circulation after intense exercise. The measured increase in albumin synthesis did not account for the entire increase in albumin content during recovery from exercise. Although the measured increase in albumin synthesis did not account for the elevated plasma albumin content in the 6 hours following exercise, we concluded that it was elevated sufficiently to account for the increased albumin content measured (in a prior study) after 24 hours of recovery.

我们从强烈的恢复过程中测量了肝白蛋白合成 以前显示的间歇性运动方案可以扩大等离子体积。 使用稳定的同位素示踪剂的底漆输注 苯丙氨酸，肝分数合成速率（FSR）和绝对 白蛋白的合成速率（ASR）是根据富集确定的 白蛋白和自由血管内前体库中的苯丙氨酸。 白蛋白FSR和ASR为6.39 +0.48％1和120 +9mg（kgbw）-1 day-1， 分别在3到6小时后恢复运动； FSR和ASR ON 时间控制学习日为5.94+0.47％第1天和104+9mg（kgbw）-1 分别分别是第1天。 FSR和ASR中的6％和16％增加（p <0.05） 运动后与血浆白蛋白含量升高有关 在5和6小时恢复时（p <0.05），总蛋白质含量增加 在整个恢复过程中（p <0.05）和负自由水清除率（p <0.05） 与基线值相比，在2,3和6.5小时的恢复时； 这些 在时间控制研究中，它们的基准不变，变量没有变化 天。 增加白蛋白含量并减少自由水的清关 强烈后，有助于在循环中保留流体 锻炼。 测得的白蛋白合成的增加没有解释 锻炼恢复过程中白蛋白含量的全部增加。 尽管测得的白蛋白合成的增加没有解释 运动后6小时，血浆白蛋白含量升高，我们 得出结论，它已足够升高以解释增加 恢复24小时后，（在先前的研究中）测量了白蛋白含量。