CHEMICAL, STRUCTURAL, AND MORPHOLOGICAL STUDIES ON CALCIUM PHOSPHATES

磷酸钙的化学、结构和形态研究

• 批准号: 2572230
• 负责人: E D EANES
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2572230
• 关键词: X ray crystallography; analytical chemistry; apatites; calcification; calcium phosphate; carbonates; chemical kinetics; chemical structure function; chromatography; electron microscopy; extracellular matrix; fluorine; liposomes; magnesium; normal ossification; phosphonate; physical chemical interaction; thermodynamics; tissue /cell culture

The purpose of this project is to study the physical, chemical, and ultrastructural properties of calcium phosphate salts, and to clarify the kinetic and thermodynamic processes and the interactions with substances of biological interest that uniquely enable calcium phosphate salts to carry out their specialized role in vivo. The properties of calcium phosphate salts are being studied with a variety of ultrastructural and physical-chemical techniques such as electron microscopy, x-ray diffraction, surface area analyses, chromatographic and standard analytical chemistry procedures. A principal endeavor currently being pursued involves artificial lipid vesicles (liposomes) as in vitro models to investigate physico- chemical aspects of matrix vesicle (MV)-mediated calcification in vivo. The latest phase of this endeavor is a study that is examining the effect that organic phosphonates have on mineral development in the liposomal model system. The aim of this study is to better delineate the physicochemical basis for the observed suppressive effects bisphosphonates have on biological calcification. Another current effort under this project is to study the physicochemical effects biologically important ions such as fluoride, carbonate, and magnesium have on the texture, i.e., size/shape of apatite crystals grown in vitro under constant physiological-like solution conditions. The aim of this study is to distinguish the direct effect fluoride has on the texture of biological apatites from that brought about by the metabolic and matrix changes this ion induces in vivo.

该项目的目的是研究物理，化学和 磷酸钙盐的超微结构特性，并澄清 动力学和热力学过程以及与 独特地启用钙的生物学兴趣的物质 磷酸盐盐在体内发挥其专业作用。 这 用A研究了磷酸钙盐的特性 多种超微结构和物理化学技术，例如 电子显微镜，X射线衍射，表面积分析， 色谱和标准分析化学程序。 一个 目前正在追求的主要努力涉及人造脂质 囊泡（脂质体）作为体外模型，以研究物理 基质囊泡（MV）介导的钙化的化学方面 体内。 这项工作的最新阶段是一项正在研究的研究 有机磷酸盐对矿物质发育的影响 脂质体模型系统。 这项研究的目的是更好 描述观察到的抑制性的物理化学基础 双膦酸盐对生物钙化的作用。 其他 该项目下的当前努力是研究物理化学 影响生物学上重要的离子，例如氟化物，碳酸盐和 镁具有质地，即磷灰石晶体的大小/形状 在恒定的生理样溶液条件下体外生长。 这项研究的目的是区分直接效应氟化物 具有由 代谢和基质改变了该离子在体内诱导。