CUMULATIVE TRAUMA DISORDER--SKELETAL MUSCLE DYSFUNCTION

累积性创伤障碍——骨骼肌功能障碍

• 批准号: 2546499
• 负责人: WILLIAM T STAUBER
• 金额: $ 19.65万
• 依托单位: WEST VIRGINIA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-30 至 1999-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2546499
• 关键词: albumins; collagen; connective tissue; dystrophin; enzyme linked immunosorbent assay; exercise; extracellular matrix; fatigue; fibrosis; fibrous protein; immunocytochemistry; laboratory rat; muscle disorders; muscle function; myofibrils; myosins; proteoglycan; striated muscles; trauma

Chronic pain originating from the musculoskeletal system is a dominant cause of sick-leave in modern industry and can be a very disabling and troublesome condition for the individual. Although the cause of this problem in skeletal muscle is unknown, one of the most frequent situations in which muscle pain is experienced is in industrial workers who have to move repeatedly and/or forcibly. The cumulative trauma disorder (CTD) which results from repetitive movements is of special interest because these repeat-motion injuries are one of the most difficult to anticipate and prevent. Our studies in humans have shown that exposure to a single bout of repeated strains can lead to myofiber and fascial rupture without bleeding but accompanied by muscle pain, restricted motion, and loss of strength and power. Little is known about the effect of repeated strains on muscles or the dynamic components of repeated use such as velocity and acceleration which produce injury resulting in CTD or CTD risk. Since variations in human exposure and response together with the necessity for repeated tissue sampling makes man unsuitable as a research subject, we have developed a rodent model of repeated strain injury (CTD). Using this model, the present study is designed: 1) to determine the dynamic factors (velocity, acceleration and dose) which produce dysfunctional versus adaptive muscles, 2) to document changes in the extracellular matrix and myofibers which lead to a pathologic muscle, and 3) to study the functional outcome and reversibility of repeated injury at different speeds and accelerations commonly experienced by hand- intensive industrial jobs. This research consists of experiments in which muscles are chronically injured by mechanical overloading in deeply anesthetized rats. The tissues are surveyed at various time intervals by biochemical, immunohistochemical and histological techniques for specific cellular markers, components and mediators involved in tissue injury and repair. The functional outcome of repeated injury is assessed by in vivo dynamometry; strength, endurance and stiffness are good measures of muscle performance. Insight into the dynamic factors producing muscle injury should provide a better understanding of the healing (adaptive) or failed-healing (pathologic) processes of muscle and aid in the design of preventative regimens for individuals in specific industrial settings. The long range goals are to determine: 1) if diminished muscle shock absorption is an impOrtant step in the development of clinical CTD; 2) if prevention of CTD can be implemented by behavioral alterations.

起源于肌肉骨骼系统的慢性疼痛是主要的 现代工业中生病的原因，可能是一个非常残疾的原因 个人的麻烦状态。虽然原因是 骨骼肌的问题未知，是最常见的问题之一 肌肉疼痛经历的情况在于工业工人 他们必须反复行动和/或强行移动。 累积创伤 由重复运动引起的障碍（CTD）是特殊的 兴趣，因为这些重复损伤是最大的一次 难以预测和预防。 我们在人类的研究表明，暴露于一次 反复的菌株会导致肌纤维和筋膜破裂而无需 出血，但伴随着肌肉疼痛，运动受限和丧失 力量和力量。关于重复菌株的影响知之甚少 关于肌肉或反复使用的动态组成部分，例如速度和 导致CTD或CTD风险的加速度。 自从 人类暴露和反应的变化以及必要 重复的组织抽样使人不适合作为研究主题，我们 已经开发了重复应变损伤（CTD）的啮齿动物模型。 使用此模型，本研究的设计：1）确定 产生的动态因素（速度，加速度和剂量） 功能失调与自适应肌肉，2）记录变化的变化 细胞外基质和肌纤维，导致病理肌肉，并且 3）研究重复损伤的功能结果和可逆性 以不同的速度和加速度通常经历 密集的工业工作。这项研究由实验组成 肌肉通过深入的机械超载长期受伤 麻醉大鼠。 以各种时间间隔对组织进行调查 通过生化，免疫组织化学和组织学技术 参与组织的特定细胞标记，组件和介体 伤害和维修。评估重复损伤的功能结果 通过体内动力学；力量，耐力和刚度很好 肌肉表现的度量。 深入了解产生肌肉损伤的动态因素应提供 更好地理解治疗（自适应）或失败 （病理）肌肉的过程和有助于预防的设计 特定工业环境中个人的方案。 远距离 目标是确定：1​​）如果吸收肌肉减少是一个 临床CTD发展的重要步骤； 2）如果预防 CTD可以通过行为改变来实现。