Cumulative Trauma Disorder: Skeletal Muscle Dysfunction

累积性创伤障碍：骨骼肌功能障碍

• 批准号: 6594711
• 负责人: WILLIAM T STAUBER
• 金额: $ 29.2万
• 依托单位: WEST VIRGINIA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-30 至 2004-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6594711
• 关键词: bradykinin; collagen; high performance liquid chromatography; immunocytochemistry; laboratory rat; muscle function; occupational hazard; pathologic process; repetitive motion injury; striated muscles; transforming growth factors; western blottings

DESCRIPTION: Chronic pain originating from the musculoskelatal system is a dominant cause of sick-leave in modem industry and can be a very disabling and troublesome condition for the individual. Although the cause of this problem in skeletal muscle is unknown, one of the most frequent situations in which muscle pain is experienced is in industrial workers who have to move repeatedly and/or forcibly. The cumulative trauma disorder (CTD) which results from repetitive movements is of special interest because these repeat-motion injuries are one of the most difficult to anticipate and prevent. Our studies in humans have shown that exposure to a single bout of repeated strains can lead to myofiber and fascial rupture without bleeding but accompanied by muscle pain, restricted motion, and loss of strength and power. Little is known about the effect of repeated strains on muscles or the dynamic components of repeated use such as velocity and acceleration, which produce injury resulting in CTD or CTD risk. Since variations in human exposure and response together with the necessity for repeated tissue sampling makes man unsuitable as a research subject, we have developed a rat model of repeated strain injury (CTD). Using this model, the present study is designed: (1) to produce muscle strain injury in rats with and without fatigue using a custom-built dynamometer which can result in pathologic fibers, (2) to measure TGF-beta and bradykinin in response to muscle injury with and without pathology, (3) to assess the role of TGF-beta in collagen production and 4) to assay for TGF-beta in the blood of strain injured rats. Pathologic muscle fibers associated with TGF-beta will be identified using immunohistochemical techniques. Collagen content will be measured by HPLC. Active and latent forms of TGF-beta 1 and TGF-beta2 and type I and III collagens will be quantified by Western blots. Insight into the factors and conditions producing muscle injury with pathology should provide a better understanding of the healing (adaptive) or failed-healing (pathologic) processes of muscle and aid in the design of preventative regimens for individuals in specific industrial settings.

描述：起源于肌肉乳突系统的慢性疼痛是一个 现代行业中病假病的主要原因，可能是一个非常残疾的原因， 个人的麻烦状态。虽然是此问题的原因 骨骼肌未知，是肌肉最常见的情况之一 疼痛是经历的，这些工人必须反复行动和/或 强制。累积创伤障碍（CTD），由重复性产生 运动是特别感兴趣的，因为这些重复损伤是一个 最难预测和预防的是。 我们在人类的研究表明，暴露于一次重复的一回合 菌株会导致肌纤维和筋膜破裂而不会流血，但是 伴随着肌肉疼痛，运动受限以及力量和力量的丧失。 关于重复菌株对肌肉或动态的影响知之甚少 重复使用的组件，例如速度和加速度，产生 受伤导致CTD或CTD风险。由于人类暴露和 响应以及重复组织采样的必要性使人 作为研究主题不适合，我们开发了一个重复的大鼠模型 应变损伤（CTD）。 使用此模型，本研究的设计：（1）产生肌肉应变 使用定制的测功机，有和不疲劳的大鼠受伤 可以导致病理纤维，（2）测量TGF-beta和Bradyinin 对有或没有病理的肌肉损伤的反应，（3）评估 胶原蛋白产生的TGF-β，4）分析TGF-beta的血液 菌株受伤的大鼠。与TGF-β相关的病理肌纤维将是 使用免疫组织化学技术鉴定。胶原蛋白的含量将是 通过HPLC测量。 TGF-BETA 1和TGF-BETA2的主动和潜在形式和类型 I和III胶原蛋白将通过Western印迹进行量化。 洞悉因病理而产生肌肉损伤的因素和条件 应该更好地了解治疗（自适应）或 肌肉的治疗失败（病理）过程，并有助于设计 在特定工业环境中个人的预防疗法。