A Facility to Generate Connectomics Information

生成连接组学信息的工具

基本信息

批准号：
10596124
负责人：
Jeff W Lichtman
金额：
$ 126.22万
依托单位：
HARVARD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-30 至 2025-02-28
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10596124
关键词：
Address Brain Brain Diseases Cells Classification Communities Computer Assisted Computer software Data Data Set Devices Educational workshop Electron Microscope Electron Microscopy Electron Microscopy Facility Electronic Mail Environment Esthetics Fees Funding Goals Heavy Metals Human Resources Image Intuition Journals Laboratories Learning Maps Methods Microtome - medical device Minor Nervous System Nervous System Physiology Neuroglia Neurons Neurosciences Organoids Persons Plant Resins Positioning Attribute Process Production Property Publications Reporting Research Personnel Resolution Resources Roentgen Rays Scanning Scheme Services Silicon Site Visit Speed Stains Structure Synapses Techniques Technology Thinness Tissue Sample Tissues Training United States National Institutes of Health Voice Work Writing X-Ray Computed Tomography brain tissue cell type improved instrument interest large datasets nanoscale neural circuit neuronal circuitry new technology outreach sample fixation symposium tissue preparation tool

项目摘要

The goal of this proposal is to disseminate high resolution large volume serial section electron microscopy data to neuroscientists. Using our electron microscopy facility, we will provide user training on the use of these new technologies and provide access to our specialized facilities so that they may generate permanent ultrathin sections and create data sets amenable for neural circuit analysis or neural and glial cell type analysis. We will make minor improvements in our software to increase the scale and efficiency of user data production to meet the needs of a user community that are inexperienced in these approaches. This project addresses compelling needs of neuroscience researchers who work on the first high priority goal of the BRAIN 2025 report: “Mapping the Structure and Components of Circuits” by providing them access to tools that are otherwise unavailable or impractical in their current form.

该提案的目标是传播高分辨率大体积串行截面电子使用我们的电子显微镜设备，我们将为用户提供显微镜数据。有关使用这些新技术的培训，并提供使用我们的专业设施的机会，以便他们可以生成永久的超薄切片并创建适合神经网络的数据集电路分析或神经和神经胶质细胞类型分析我们将在我们的分析中做出微小的改进。软件可提高用户数据生产的规模和效率，以满足用户的需求该项目解决了对这些方法缺乏经验的用户社区。致力于 BRAIN 2025 第一个高度优先目标的神经科学研究人员的需求报告：通过为他们提供工具来“绘制电路的结构和组件” 以目前的形式无法获得或不切实际。

项目成果

期刊论文数量（3）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Volume Electron Microscopy Workflows for the study of Large-Scale Neural Connectomics.

用于研究大规模神经连接组学的体积电子显微镜工作流程。

DOI：
发表时间：
2023-07-22
期刊：
影响因子：
0
作者：
Schalek, R L;Lu, X;Petkova, M;Boulanger;Karlupia, N;Wu, Y;Wang, S;Wang, X;Dhanyasi, N;Berger, D;Han, X;Sjostedt, E;Engert, F;Lichtman, J W
通讯作者：
Lichtman, J W

Connectomics of the Octopus vulgaris vertical lobe provides insight into conserved and novel principles of a memory acquisition network.

普通章鱼垂直叶的连接组学提供了对记忆获取网络的保守和新颖原理的见解。

DOI：
发表时间：
2023-07-06
期刊：
影响因子：
7.7
作者：
Bidel, Flavie;Meirovitch, Yaron;Schalek, Richard Lee;Lu, Xiaotang;Pavarino, Elisa Catherine;Yang, Fuming;Peleg, Adi;Wu, Yuelong;Shomrat, Tal;Berger, Daniel Raimund;Shaked, Adi;Lichtman, Jeff William;Hochner, Binyamin
通讯作者：
Hochner, Binyamin

A molecular filter for the cnidarian stinging response.

用于刺胞动物刺痛反应的分子过滤器。