Identification of bioactive compounds from tef (Eragrostis tef) seed extracts for antioxidant properties.

从 tef（画眉草 tef）种子提取物中鉴定生物活性化合物的抗氧化特性。

基本信息

批准号：
10553205
负责人：
Ayalew Ligaba Osena
金额：
$ 14.2万
依托单位：
UNIVERSITY OF NORTH CAROLINA GREENSBORO
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-02-01 至 2024-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10553205
关键词：
Acids Adult Affect Africa Age Alternative Medicine Anabolism Anthocyanins Antioxidants Australia Barley Biochemical Biological Assay Blood Glucose Bread Cardiovascular Diseases Celiac Disease Cell model Cells Cereals Chemicals China Colon Consumption Country Cytoprotection DNA Shuffling Data Diabetes Mellitus Diet Dietary Fiber Disease Environment Eragrostis Eritrea Essential Amino Acids Ethiopia Female Flavonoids Food Funding Genes Genetic Genetic Variation Genome Genotype Glutamate-Cysteine Ligase Glutathione Glutathione Reductase Gluten Grain Health Health Benefit Histidine Horns India Inflammatory Intake Investments Knowledge Laboratories Letters Linoleic Acids Lipid Peroxidation Luciferases Lysine Malignant Neoplasms Mammalian Cell Membrane Lipids Methionine Millet Minerals Minority Groups Modeling Molecular Genetics National Research Council Non-Insulin-Dependent Diabetes Mellitus Nutrient Nutritional Oxidative Stress Oxidative Stress Induction Oxidoreductase Pathogenicity Pathway interactions Persons Phenols Physiological Phytochemical Play Polyunsaturated Fatty Acids Population Production Property Quinones Reactive Oxygen Species Recommendation Reporter Genes Research Resistance Risk Factors Role Rye cereal Signal Transduction South Africa Starch Sulfonic Acids Techniques Testing Trace Elements United States United States National Institutes of Health Variant Water-Soluble Vitamin Weight maintenance regimen Wheat combat diphenyl food security heme oxygenase-1 human disease immunoreaction improved macromineral male monocyte novel nuclear factor-erythroid 2 polyphenol prevent response stable cell line student training trait transcriptome underrepresented minority student

项目摘要

Summary Cellular oxidative stress is a risk factor for several human disease including diabetes, cardiovascular diseases (CVD) and cancer. Natural and food products have a great potential to protect cells under oxidative stress conditions. Tef (Eragrostis tef) is gluten-free grain that is rich in mineral nutrients, essential amino acids, and water-soluble vitamins and several bioactive phytochemicals including polyphenols, flavonoids and polyunsaturated fatty acids (PUFAs). Tef has been recognized by the U.S. National Research Council as a crop with excellent nutritional potential and suitability to boost food security. However, the bioactivity of tef phytochemicals have never been studied using physiologically relevant models. In this study, we propose to identify bioactive compounds in tef grains and test their ability to combat oxidative stress, thus preventing the pathogenic conditions associated with reactive oxygen species (ROS)-induced damages. This initiative is based on our recent finding indicating that: 1) tef seed extracts increase glutathione (GSH) levels in THP-1 monocytes, 2) an increase in GSH level is more prominent in brown than ivory tef seeds, suggesting the existence of genotypic variability in the content of bioactive compound, 3) Tef seed extracts increase the expression of the master regulator of antioxidant pathway nuclear factor erythroid 2-related factor 2 (Nrf2) and its targets including Heme Oxygenase 1 (HO-1), NAD(P)H quinone oxidoreductase1 (NQO1), glutathione reductase (GR) and the γ-glutamate cysteine ligase catalytic (GCLC) and regulatory (GCLM) subunit, which play a key role in GSH biosynthesis, 4) A bioactive fraction of tef extract contains a derivative of linoleic acid (LA) 9-Hydroxyoctadecadienoic acid (9-HODE), which is a marker for membrane lipid peroxidation. These findings prompted us to hypothesize that tef has bioactive compounds which have the potential to boost the antioxidant pathway under oxidative stress conditions likely via Nrf2 signaling. Our aims are: 1) Identification and functional characterization of bioactive phytochemicals from tef seeds, 2) Studying whether tef bioactive compounds induce oxidative stress-responsive genes, and protect cells from ROS, 3) Understanding the genetic mechanism regulating antioxidant properties of tef. This study will help in developing alternative medicines for oxidative stress-induced diseases. It will also generate new information that can be used to improve tef or transferred to major cereals to add value to these crops. This funding will boost the capacity of the Osena laboratory to explore underutilized crops such as millets for desirable traits including health benefits. The Osena laboratory and the environment at UNC Greensboro are ideal for this research, and training students from underrepresented minority group.

概括细胞氧化应激是多种人类疾病的危险因素，包括糖尿病、天然食品和食品具有治疗心血管疾病（CVD）和癌症的巨大潜力。 Tef（Eragrostis tef）是一种富含无麸质谷物，可在氧化应激条件下保护细胞。富含矿物质营养素、必需氨基酸、水溶性维生素和多种生物活性物质植物化学物质包括多酚、类黄酮和多不饱和脂肪酸（PUFA）。被美国国家研究委员会认定为营养优良的作物然而，tef 植物化学物质的生物活性具有促进粮食安全的潜力和适用性。从未使用生理相关模型进行研究，在这项研究中，我们建议确定。 TEF 谷物中的生物活性化合物并测试其对抗氧化应激的能力，从而预防与活性氧（ROS）引起的损伤相关的致病条件。该举措基于我们最近的发现，表明：1) tef 种子提取物可增加谷胱甘肽 THP-1 单核细胞中的 (GSH) 水平，2) 棕色细胞中 GSH 水平的增加更为显着象牙 tef 种子，表明生物活性物质含量存在基因型变异化合物，3) Tef 种子提取物增加抗氧化剂主调节剂的表达途径核因子红细胞 2 相关因子 2 (Nrf2) 及其靶标（包括血红素）加氧酶 1 (HO-1)、NAD(P)H 醌氧化还原酶 1 (NQO1)、谷胱甘肽还原酶 (GR) 以及γ-谷氨酸半胱氨酸连接酶催化（GCLC）和调节（GCLM）亚基，其作用在 GSH 生物合成中发挥关键作用，4) tef 提取物的生物活性部分含有亚油酸衍生物酸 (LA) 9-羟基十八碳二烯酸 (9-HODE)，是膜脂的标记物这些发现促使我们发现 tef 具有生物活性化合物。在氧化应激条件下有可能增强抗氧化途径我们的目标是：1) 生物活性的鉴定和功能表征。来自 tef 种子的植物化学物质，2) 研究 tef 生物活性化合物是否诱导氧化应激反应基因，并保护细胞免受ROS的侵害，3）了解遗传机制调节 tef 的抗氧化特性这项研究将有助于开发替代药物。它还将产生可用于氧化应激诱发的疾病的新信息。提高 TEF 或转移到主要谷物以增加这些作物的价值。奥塞纳实验室探索未充分利用的作物（如小米）以获得理想性状的能力包括北卡罗来纳大学格林斯博罗分校的奥塞纳实验室和环境。非常适合这项研究，并培训来自代表性不足的少数群体的学生。