The Human Lung BioMolecular Multi-Scale Atlas Program (HuBMAP-Lung)

人肺生物分子多尺度图谱计划 (HuBMAP-Lung)

• 批准号: 10530972
• 负责人: GLORIA S PRYHUBER
• 金额: $ 225万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10530972
• 关键词: 3-Dimensional; Adult; Alveolus; Anatomy; Atlases; Biological Assay; Biology; California; Cell Nucleus; Cell physiology; Cells; Chromatin; Collaborations; Collection; Communication; Communities; Coordination and Collaboration; Data; Data Analyses; Data Collection; Disease; Education; Educational workshop; Elements; Experimental Designs; FAIR principles; Gene Expression; Goals; Human; Human BioMolecular Atlas Program; Image; Institution; International; Knowledge; Laboratories; Leadership; Learning; Lipids; Location; Lung; Lung diseases; Maps; Microscopy; Molecular; Names; Nomenclature; North Carolina; Organ; Pacific Northwest; Phase; Pilot Projects; Post-Translational Protein Processing; Production; Productivity; Proteins; Protocols documentation; Public Health; Recording of previous events; Research; Research Personnel; Resolution; Resource Sharing; Resources; Respiratory System; Sensitivity and Specificity; Standardization; Structure; Structure of parenchyma of lung; Technology; Therapeutic Intervention; Trachea; United States National Institutes of Health; Universities; Visualization; Washington; body system; cell type; clinical care; collaboratory; data management; data portal; data sharing; expectation; experience; improved; indexing; interest; interoperability; lung injury; meetings; member; multi-scale atlas; new technology; novel strategies; programs; pulmonary function; repaired; success; tissue mapping; tissue resource; transcriptome sequencing; transcriptomics; working group

Project Summary/Abstract Overall Research Plan The Human Lung Biomolecular Multi-Scale Atlas Program (HuBMAP-Lung) The Human Biomolecular Atlas Program (HuBMAP) is entering the production phase, with an emphasis on progressive, high sensitivity and specificity assays, prioritizing spatial biomolecular analysis. Our Tissue Mapping Center (TMC) proposal will focus on one organ system, comprised of two lungs and related respiratory tract (trachea to alveoli) that for simplicity we refer to as the “Lung”. Our TMC will generate, standardize, and validate extensive data from high content, high-throughput imaging and `omics technology to produce systematic human lung tissue maps at high resolution. We will take a long view of an Atlas, as a collection of 2D and 3D maps containing images, tabular data, facts about multiple locations at varying resolution, and indexes of named objects keyed to coordinates of a locational grid analogous to latitude and longitude, to cartographically present the whole range of salient features of the human Lung. To accomplish this task, our center brings together Investigators from five institutions, the University of Rochester (URMC), University of California at San Diego (UCSD), Pacific Northwest National Laboratory (PNNL), University of Washington (UW), and University of North Carolina at Chapel Hill (UNC), each with complementary lung-focused academic interests, knowledge, and inter- consortia network connections and with internationally recognized expertise in lung and state-of-the-art biomolecular technologies. Our Lung data provided to the HuBMAP Data Portal in Phase I has been focused to single nucleus RNA-sequencing, chromatin availability and beginning spatial transcriptomics. In addition to further increasing representation of human diversity in these data types, the next phase of our TMC turns primary effort toward determining spatial organization of cells and matrices defined by not only gene expression but also proteins, protein modifications, lipids and select metabolites critical to specific cell function within anatomical and functional niches. With the recognized value of global investigative efforts within and outside the Consortium, the Specific Aims of the TMC Overall Component concentrate on Communication and Collaboration within the TMC, between all HuBMAP components and synergistically among national and international researchers and Consortia.

项目概要/摘要 总体研究计划 人肺生物分子多尺度图谱计划 (HuBMAP-Lung) 人类生物分子图谱计划 (HuBMAP) 正在进入生产阶段，重点是 先进、高灵敏度和特异性的检测，优先考虑我们的组织图谱分析。 中心（TMC）提案将重点关注一个器官系统，由两个肺和相关呼吸道组成 （气管到肺泡），为简单起见，我们将其称为“肺”，我们的 TMC 将生成、标准化和验证。 来自高内涵、高通量成像和组学技术的广泛数据，可产生系统的人类 我们将以 2D 和 3D 地图的集合形式查看 Atlas 的长视图。 包含图像、表格数据、不同分辨率下多个位置的事实以及命名索引 以类似于纬度和经度的位置网格坐标为关键的对象，以地图形式呈现 为了完成这项任务，我们的中心汇集了人类肺的全部显着特征。 来自罗切斯特大学（URMC）、加州大学圣地亚哥分校等五家机构的研究人员 (UCSD)、太平洋西北国家实验室 (PNNL)、华盛顿大学 (UW) 和北方大学 卡罗莱纳大学教堂山分校 (UNC)，每个人都具有互补的以肺为中心的学术兴趣、知识和互动 联盟网络连接以及国际公认的肺和最先进的专业知识 我们在第一阶段向 HuBMAP 数据门户提供的肺部数据主要用于 单核 RNA 测序、染色质可用性和起始空间转录组学。 进一步增加这些数据类型中人类多样性的代表性，我们 TMC 的下一阶段将成为主要阶段 努力确定细胞和基质的空间组织，不仅由基因表达定义，还由基因表达定义 蛋白质、蛋白质修饰、脂质和选择代谢物对解剖和细胞内的特定细胞功能至关重要 凭借联盟内外全球调查工作的公认价值， TMC 整体组件的具体目标集中于内部的沟通和协作 TMC，在所有 HuBMAP 组成部分之间以及国内和国际研究人员之间的协同作用 财团。