Biomedical Research Core

生物医学研究核心

基本信息

批准号：
10707954
负责人：
GLORIA S PRYHUBER
金额：
$ 12.1万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-09-21 至 2027-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10707954
关键词：
Acceleration Adolescent Adopted Adult Age Aging Atlases Authorization documentation Biological Assay Biology Biomedical Research Biopsy Cell Line Cells Cessation of life Child Childhood Clinical Collaborations Communities Consent Data Development Distal Drug toxicity Early Diagnosis End stage renal failure Engineering Ensure FAIR principles Generations Gifts Goals Human Hypertension Image Incidence Infant Infrastructure Instruction Kidney Kidney Diseases Kidney Failure Knowledge Life Longevity Measurement Methods Molecular Morphology Nature Neonatal Nephrology Nephrons Organ Organ Transplantation Organoids Parents Pathologic Pathology Patients Pediatric Research Physiological Policies Positioning Attribute Procedures Protocols documentation Provider Publishing Quality Control Reporter Research Research Personnel Resources Sampling Severities Source Specimen Technology Time Tissue Banks Tissue Engineering Tissue Sample Tissues Translational Research Trauma United States Universities Washington authority biological research cell type delivery complications design design verification effective intervention epigenome fetal gene function induced pluripotent stem cell innovation interest interoperability kidney cell kidney dysfunction molecular imaging multimodality multiple omics operation postnatal premature preservation prevent quality assurance repository screening single cell technology tissue processing transcriptome validation studies

项目摘要

Abstract Pediatric Kidney BioMedical Core (pKidBIO Core) Kidney disease is a major cause of illness and death in infants, children, and adolescents with the incidence of end-stage kidney disease (ESKD) in these patients in the United States of about 15 per million. The rapidly increasing problems of adult hypertension and renal failure may also have roots in pediatric kidney insults that go undetected, unprevented and untreated. The development of effective interventions and methods of early detection and severity measurements of renal disease in children is lagging in part due to a lack of knowledge of physiological and pathological changes that occur as the kidney matures. Molecular blueprints would dramatically enhance our ability to design effective approaches to intervene and prevent kidney dysfunction. This goal cannot be met, however, without having a source of pediatric kidney tissue to begin molecular interrogations to identify the, uniquely human and developmental, ’omic instructions required to make and maintain healthy kidneys. The pKidBIO core, as part of the Washington University Pediatric Center of Excellence, is in an unusually strong and unique position to provide the required pediatric kidney tissues, originating from clinical biopsies or as “gifts of life” consented for research, preserved with methods shown to be compatible with the most recent technologies to enable age-specific research to dig into multi-omic single cell transcriptome and epigenome assays, molecular imaging and tissue engineering. By its design and nature, the pKidBIO core will promote enthusiasm and progress in pediatric kidney disease research by 1) providing human age-specific references for fetal and childhood kidney disease tissues, 2) enabling studies aimed to delineate cellular, morphological, physiological and molecular changes associated with postnatal kidney maturation, 3) accelerating scientific research aimed at ex vivo human kidney organoids, 4) establishing protocols for isolating differentiated kidney cell types at stages consistent with those seen in kidney tissue samples, 5) advancing drug toxicity screening, and by 6) designing validation studies of gene function and kidney engineering. The availability of the tissue and the outstanding data generated from them will attract new expertise outside kidney research developing spatial imaging and analytical technologies and research interested in physiological aging across the lifespan. The pKidBIO repository will be a national resource for the research and clinical community that is findable, accessible, interoperable, and reusable.

抽象的小儿肾脏生物医学核心 (pKidBIO Core) 肾脏疾病是婴儿、儿童和青少年疾病和死亡的主要原因在美国，这些患者的终末期肾病 (ESKD) 发病率约为 15 每百万人中快速增加的成人高血压和肾衰竭问题也可能有。其根源在于儿童肾脏损伤未被发现、预防和治疗。早期发现和严重程度测量的有效干预措施和方法儿童疾病的滞后部分原因是缺乏生理和病理知识随着肾脏成熟而发生的变化将极大地增强我们的能力。设计有效方法来干预和预防肾功能障碍的能力。然而，如果没有儿科肾组织来源来开始分子生物学研究，就无法实现这一目标。审讯以确定独特的人类和发展的“组学指令” pKidBIO 核心，作为华盛顿大学的一部分，制造并维持健康的肾脏。儿科卓越中心处于异常强大和独特的地位，可以提供所需的儿科肾组织，源自临床活检或同意作为“生命的礼物” 研究，采用与最新技术兼容的方法保存使特定年龄的研究能够深入研究多组学单细胞转录组和表观基因组根据其设计和性质，pKidBIO 核心将在分析、分子成像和组织工程方面发挥重要作用。通过 1) 提供人力来促进小儿肾脏疾病研究的热情和进步胎儿和儿童肾脏疾病组织的年龄特定参考，2) 使研究旨在描绘与产后相关的细胞、形态、生理和分子变化肾脏成熟，3) 加速针对离体人类肾脏类器官的科学研究，4) 建立与这些阶段一致的分离分化肾细胞类型的方案在肾组织样本中观察到，5) 推进药物毒性筛选，以及 6) 设计验证基因功能和肾脏工程的研究组织的可用性和突出。从它们生成的数据将吸引肾脏研究之外的新专业知识开发空间整个领域对生理衰老感兴趣的成像和分析技术以及研究 pKidBIO 存储库将成为研究和临床的国家资源。可查找、可访问、可互操作和可重用的社区。