Breathprinting as a window into gut microbiome chemoecology

呼吸印迹是了解肠道微生物组化学生态学的窗口

• 批准号: 10512356
• 负责人: Andrew Leon Kau
• 金额: $ 73.47万
• 依托单位: CHILDREN'S HOSP OF PHILADELPHIA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2027-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10512356
• 关键词: Affect; Age; Age-Months; Anabolism; Anaerobic Bacteria; Animal Husbandry; Antibiotic Therapy; Antibiotics; Autoimmunity; Bacteria; Bacteroides fragilis; Biological; Biological Markers; Birth; Breath Tests; COVID-19; Catabolism; Catalogs; Characteristics; Childhood; Chronic lung disease; Clinical; Clinical Research; Cohort Studies; Commerce; Data; Development; Diagnosis; Diagnostic; Diagnostic tests; Diet; Disease; Disulfides; Ecology; Enrollment; Ensure; Environmental Exposure; Evaluation; Exhalation; Fever; Food Processing; Genetic Transcription; Genetic study; Germ-Free; Gnotobiotic; Goals; Health; Heart Diseases; Human; Immune system; Immunity; In Vitro; Individual; Infant; Investigation; Knowledge; Link; Logistics; Lyase; Malignant Neoplasms; Malnutrition; Measurement; Measures; Medical; Metabolic; Metabolic Pathway; Metabolism; Metagenomics; Methionine; Methodology; Molecular; Mus; Nervous System Physiology; Patient Care; Pattern; Pharmaceutical Preparations; Population; Prospective cohort; Public Health; Research; Sampling; Specimen; System; Technology; Testing; Therapeutic; Time; Work; bacterial metabolism; base; breath composition; clinical diagnostics; cohort; experience; fecal microbiota; gut health; gut microbes; gut microbiome; gut microbiota; human disease; improved; infancy; innovation; member; metagenomic sequencing; metatranscriptomics; microbial; microbiome research; mouse model; novel strategies; point of care testing; response; success

PROJECT SUMMARY The gut microbiome has a profound effect on human health by influencing the development of the immune system, producing bioactive metabolites and directly aiding in the processing of food. Changes in the composition and metabolism of the gut microbial community has been associated with a wide array of human diseases, including malnutrition, auto-immunity, and even neurological function. A central aspiration of gut microbiome research is to identify individuals with abnormalities in gut microbial composition in order to intervene therapeutically. However, a major limitation is that it is logistically challenging to evaluate gut microbial composition in a patient-care setting. The studies in this proposal will advance a novel strategy to overcome this roadblock. Based on powerful preliminary studies in germ-free mice and in humans, our central hypothesis is that the volatile composition of breath offers a dynamic representation of the composition and function of the gut microbiota. We will test this hypothesis by evaluating the association between breath, gut bacterial species, and microbial metabolic functions (Aim 1). In addition, we will use two unique clinical cohorts of infants to determine how the breath volatile profile changes dynamically with changes in gut microbial community composition (Aim 2). Together, our studies will not only advance our fundamental understanding of the spectrum of breath volatiles that arise from microbial metabolism, but will also be a critical first step in determining how “breathalyzer” diagnostics of gut health can be applied clinically.

项目概要 肠道微生物组通过影响免疫系统的发育对人类健康产生深远影响 系统，产生生物活性代谢物并直接帮助食品加工。 肠道微生物群落的组成和代谢与多种人类 疾病，包括营养不良、自身免疫，甚至神经功能。 微生物组研究是为了识别肠道微生物组成异常的个体，以便进行干预 然而，一个主要的限制是评估肠道微生物在逻辑上具有挑战性。 本提案中的研究将提出一种克服这一问题的新策略。 基于对无菌小鼠和人类的强有力的初步研究，我们的中心假设是 呼吸的挥发性成分提供了肠道成分和功能的动态表征 我们将通过评估呼吸、肠道细菌种类和微生物之间的关联来检验这一假设。 此外，我们将使用两个独特的婴儿临床队列来确定微生物代谢功能（目标 1）。 挥发性呼吸特征如何随着肠道微生物群落组成的变化而动态变化（目标 2). 我们的研究不仅将增进我们对呼吸挥发物谱的基本了解。 这是由微生物新陈代谢产生的，但也将是确定“呼吸分析仪”如何发挥作用的关键第一步 肠道健康的诊断可以应用于临床。