Microbial Origin of Breath Volatile Metabolites

呼吸挥发性代谢物的微生物来源

基本信息

批准号：
10171781
负责人：
Andrew Leon Kau
金额：
$ 20.84万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-06-01 至 2023-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10171781
关键词：
Anaerobic Bacteria Animal Husbandry Asthma Autoimmune Diseases Bacteria Bile Acids and Salts Biological Biological Markers Breath Tests Chemical Structure Clinical Collection Communicable Diseases Coupling Data Diagnostic Diagnostic tests Disease Ecology Ensure Exhalation Genes Germ-Free Gnotobiotic Health Human Hypersensitivity In Vitro Individual Investigation Knowledge Lung Malignant Neoplasms Mass Spectrum Analysis Mediating Metabolic Metagenomics Methods Microbe Modeling Mus Obesity Output Participant Patient Care Pattern Reproducibility Research Sampling Shapes Techniques Testing Time Translating Volatile Fatty Acids Work base breath composition dysbiosis experience gut bacteria gut dysbiosis gut microbes gut microbiota immune function improved innovation insight lipophilicity metagenomic sequencing microbial microbial community microbiota novel novel therapeutics point of care testing point-of-care diagnostics pressure success tool vapor volatile organic compound

项目摘要

ABSTRACT Gut microbial communities influence multiple facets of human health, impacting common disorders such as obesity, autoimmune diseases, and allergies. These discoveries were enabled by the application of metagenomic sequencing and mass spectrometric techniques to human fecal samples, leading to remarkable insights into the interplay between gut microbial communities and human health. However, a major barrier to translating our growing knowledge about the gut microbiota and its functions to the patient-care setting is the difficulty in obtaining, storing, processing and analyzing fecal samples. Volatile organic compounds (VOCs) are present in exhaled breath and are produced by many microbes. By coupling a new method for analyzing the VOC composition of mouse breath samples with our gnotobiotic model, we find that breath VOC patterns reflect the gut microbial communities. Based on this preliminary data, our central hypothesis is that many VOCs observed in breath are produced by the gut microbiota and thus represent biomarkers of gut microbial community configuration and function. We will test our central hypothesis through the following specific aims: (1) Evaluate the extent to which gut microbial communities shape breath VOC profiles; and (2) Characterize human gut microbes that generate VOCs and demonstrate that microbe-produced VOCs are present in the breath of gnotobiotic mice. Our investigations will be significant, because we will greatly advance our fundamental understanding of the gut microbial contribution to breath volatile composition, and we will begin to identify specific, individual volatile-microbe correlations. Our work will therefore launch a new paradigm towards novel, non-invasive “breathalyzer” diagnostics to interrogate gut microbial dysbiosis.

抽象的肠道微生物群落影响人类健康的多个方面，影响常见疾病，例如这些发现是通过应用来实现的。对人类粪便样本进行宏基因组测序和质谱技术，取得了显着的成果然而，肠道微生物群落与人类健康之间相互作用的一个主要障碍。将我们不断增长的关于肠道微生物群及其功能的知识转化为患者护理环境获取、储存、处理和分析粪便样品的困难在于挥发性有机化合物。（VOC）存在于呼出的气体中，由许多微生物通过耦合新方法产生。使用我们的无菌模型分析小鼠呼吸样本的 VOC 成分，我们发现呼吸 VOC 基于这些初步数据，我们的中心假设是：呼吸中观察到的许多 VOC 是由肠道微生物群产生的，因此代表了我们将通过以下内容检验我们的中心假设。具体目标：(1) 评估肠道微生物群落对呼吸 VOC 特征的影响程度；(2) 表征产生 VOC 的人类肠道微生物，并证明微生物产生的 VOC 我们的研究将具有重大意义，因为我们将取得巨大进展。我们对肠道微生物对呼吸挥发性成分的贡献有了基本的了解，我们将开始因此，我们的工作将推出一个新的范式，以确定特定的、个体的挥发性微生物的相关性。新颖的、非侵入性的“呼吸分析仪”诊断方法可以检查肠道是否存在微生物失调。

项目成果

期刊论文数量（1）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Developmental progression of the nasopharyngeal microbiome during childhood and association with the lower airway microbiome.

儿童期鼻咽微生物组的发育进展及其与下呼吸道微生物组的关联。

DOI：
发表时间：
2023-09-19
期刊：
影响因子：
0
作者：
Hernandez;Rosen, Anne L;Tomera, Christopher P;Lin, Elaina E;Akaho, Elikplim H;Blatz, Allison M;Otto, William R;Logan, Joey;Young, Lisa R;Harris, Rebecca M;Kau, Andrew L;John, Audrey R Odom
通讯作者：
John, Audrey R Odom