Research Services Core (RSC)

研究服务核心 (RSC)

• 批准号: 10682313
• 负责人: Samuel Michael
• 金额: $ 431.23万
• 依托单位: NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10682313
• 关键词: Acoustics; Aptitude; Archives; Automation; Biological; Biological Assay; Biology; COVID-19; Cells; Chemical Structure; Chemicals; Collaborations; Collection; Communities; Complement; Complex; Computer software; Core Facility; Custom; Data Analyses; Databases; Development; Devices; Dose; Emerging Technologies; Engineering; Ensure; Equipment; Funding; Generations; Hybrids; Incubators; Institutes; Internet; Intramural Research Program; Laboratories; Libraries; Liquid substance; Location; Maintenance; Methods; Mission; Monitor; National Center for Advancing Translational Sciences; National Institute of Allergy and Infectious Disease; Nature; Prevention program; Process; Productivity; Protocols documentation; RNA Interference; RNA interference screen; Rapid screening; Reader; Reagent; Recording of previous events; Research; Research Personnel; Research Project Grants; Research Support; Resources; Retrieval; Robotics; Running; Sampling; Schedule; Scientist; Services; Ships; Support System; System; Technology; Time; Toxicology; Training; Translations; Transportation; United States National Institutes of Health; Vial device; Viral; Work; arm; assay development; cohesion; data quality; data sharing; design; detection assay; detection method; flexibility; follow-up; high throughput screening; imager; instrument; instrumentation; member; new technology; next generation; operation; programs; response; screening; small molecule; stem cells; tissue culture; tool; vigilance

Research Services Core (RSC)staff provide support to the NCATS activities in high-throughput screening, assay development, and optimization as well as follow-up and dose-response library-plating services for NCATS screening activities. The Compound Management team works in conjunction with the Automation team to provide follow-up and dose-response library-plating services for NCATS screening activities. Systems supported by the Automation team include primary screening system, Tox21 screening system, RNAi screening system, the RNAi hit picking system for assay follow ups, and multiple LabCyte Access acoustic plating systems. In the past year, we have also installed and begun to operate a LabCyte Access system to perform high throughput screens in Building 33 in a collaboration with NIAID. Together, all these systems combined provide a wide variety of automation and compound plating tasks used to enable all facets of the NCATS intramural scientific program. The primary screening system utilizes three robotic arms for plate transportation, a storage capacity of more than 3 million compound wells (approximately 2,000 1,536-well compound plates), and more than 1.5 million assay wells (approximately 1,100 1,536-well assay plates) to achieve a world-class screening productivity while maintaining the high level of data quality required by researchers. It also can run multiple assays in parallel. Center experts have developed custom software to monitor and control the system, in addition to archiving the complete process history for every screen in real time. The Tox21 screening system uses a single robotic arm for plate transportation and a storage capacity of nearly 1 million compound or assay wells (about 600 1,536-well compound or assay plates) to screen smaller scale compound libraries not present on the primary screening system and the Tox21 compound library. This platform is designed to complement the primary system, and it is easily customizable, allowing for the rapid integration of additional screening technologies. The RSC team additionally provides screening system services to intramural programs within NCATS. The Tox21 screening system contains a single robotic arm for plate transportation and a storage capacity of about 1.5 million compound wells (about 1,000 1,536-well compound plates) and more than 1.5 million assay wells (approximately 1,100 1,536-well assay plates). With a pin tool and two acoustic dispensers for compound addition, four low-volume dispensers for reagent addition, and three plate readers enabling a variety of assay detection methods, this system can rapidly screen, in triplicate, the Toxicology in the 21st Century (Tox21) library of 10,000 compounds. NCATS experts monitor and control this system with the same custom software used for the primary screening system. The RNAi screening system has two robotic arms for plate transportation with incubators for assay plate storage and plate stackers to facilitate continuous system operation. A multichannel pipettor, low volume dispensers, an aspirator for plate washing, and two different plate readers support a wide variety of detection methods for this RNAi-focused system. RSC staff also continue to support the Stem Cell Translation Laboratory (SCTL). Various team members have provided a large amount of support with a vast array of equipment including automated tissue culture systems, flow cytometers, cell sorters, and high content imagers. Special mention should be given to the automated tissue culture platform which has established itself as a core capability for all NCATS researchers now with the demand becoming so great that another system is most likely on order. These resources require a tremendous amount of expertise to operate and maintain. In the past year, RSC has begun to act as a core facility for these technologies, enabling different biology groups to increase their capabilities. Related to the high content imagers, more high content assays were run in the past year due to the increased capabilities as compared to the previous four years combined. In the past year, we added a 3rd automated tissue culture device to further increase our capacity in this space, and have configured a 4th, more automation friendly instrument from CellTrio, that is on schedule to be delivered and installed later next year. This year also finally saw our new Compound Management space setup after construction and renovations were complete. The Compound Management group moved from their previous B2 location to C1, which was a huge effort given the sheer volume of equipment, all the while ensuring that there was continued operation. In addition to the move, several critical pieces of equipment that had been ordered years prior were finally delivered and installed, including 2 Brooks Sample Store II -20C storage systems, which can be integrated with sample handling and LabCyte Echo plating systems to provide fully automated assay plate generation. This gives NCATS incredible capacity for both compound storage and flexibility to ship assay ready plates with custom plated libraries to collaborators around the world if required. The NCATS intramural program is heavily involved in COVID-19 relief efforts across the NIH. RSC is directly involved in several of these key initiatives, providing project management, scientific and technical support across several programs. Below are a list of some of the COVID-19 projects RSC has worked on: Antiviral Prevention Program (APP) laboratory standup: NCATS received a substantial amount of funding as part of the APP, with many or responsibilities related to high throughput screening, compound management (including anti-viral library design and compound acquisition), assay development and design, and data sharing. RSC was involved in all these activities, helping to fit out an entire lab worth of equipment including instrument selection, ordering, setup, and operation. This was a huge amount of work given the sheer volume of equipment, and in working closely with NCATS building services stood up an entire lab to initiate work for APP in a matter of months.

研究服务核心（RSC）的工作人员为NCATS活动提供了支持，在高通量筛查，测定开发和优化以及NCATS筛选活动的后续摄入和剂量响应图书馆拼写服务方面为NCATS活动提供了支持。复合管理团队与自动化团队合作，为NCATS筛选活动提供后续式和剂量响应图书馆拼图服务。由自动化团队支持的系统包括初级筛选系统，TOX21筛选系统，RNAi筛选系统，用于测定后续的RNAi命中式接收系统以及多个Labcyte访问声音电镀系统。在过去的一年中，我们还安装并开始操作一个Labcyte访问系统，以与Niaid合作，在33号建筑物中执行高吞吐量屏幕。 所有这些系统共同提供了多种自动化和复合镀金任务，用于实现NCAT的所有方面。 主要筛选系统利用三个机器人臂进行板盘运输，超过300万种化合物井（约2,000个1,536孔 - 孔化合物板）和超过150万的测定井（大约1,100 1,100 1,100 1,100 1,536孔测定板），以实现世界化筛选生产力，同时维持研究质量的高水平的研究质量。它还可以并行运行多个测定法。中心专家开发了自定义软件来监视和控制系统，除了实时归档每个屏幕的完整过程历史记录外。 TOX21筛选系统使用单个机器人臂进行板运输，并且存储容量近100万种化合物或测定井（约600 1,536孔化合物或测定板）来筛选不存在的较小的比例化合物库，而不是主要的筛选系统和TOX21复合库。该平台旨在补充主要系统，并且易于自定义，从而可以快速整合其他筛选技术。 RSC团队还为NCAT内的壁内计划提供筛查系统服务。 TOX21筛选系统包含一个用于板运输的机器人臂，存储容量约为150万种化合物井（约1,000 1,536孔复合板），超过150万种测定井（约1,100 1,100 1,536孔测定的分析板）。使用PIN工具和两个用于化合物添加的声学分配器，四个用于添加试剂的低量分配器，以及三个读取器启用各种测定检测方法的板块读取器，该系统可以迅速筛选，一式三份，21世纪的毒理学（TOX21）的毒理学（TOX21）库为10,000种化合物。 NCATS专家使用用于主要筛选系统的相同自定义软件来监视和控制此系统。 RNAi筛选系统具有两个机器人臂，用于与孵化器的板运输，用于测定板块存储和板块堆叠器，以促进连续的系统操作。多通道移液器，低体积分配器，用于板板洗涤的抽吸机和两个不同的板读取器支持此以RNAI为中心的系统的多种检测方法。 RSC工作人员还继续支持干细胞翻译实验室（SCTL）。各个团队成员都提供了大量支持，包括大量的设备，包括自动组织培养系统，流式细胞仪，细胞分类器和高内容成像仪。 应特别提及自动化组织培养平台，该平台已确立了现在的所有NCAT研究人员的核心能力，需求变得如此巨大，以至于另一个系统很可能是秩序的。 这些资源需要大量的专业知识才能运营和维护。在过去的一年中，RSC已开始充当这些技术的核心设施，使不同的生物学小组能够提高其能力。 与较高的内容成像仪相关，由于功能增加，与前四年的总和相比，过去一年中进行了更多的高内容测定。 在过去的一年中，我们添加了第三个自动化组织培养设备，以进一步提高我们在该领域的容量，并配置了Celltrio的第四次，更友好的仪器，该工具按计划进行，并在明年晚些时候安装。 在施工和翻新完成后，今年还看到了我们新的化合物管理空间设置。 化合物管理小组从以前的B2位置转移到C1，这是一项巨大的努力，鉴于设备的幅度庞大，一直确保继续操作。 除了此举外，最终交付和安装了几年前已订购的几个关键设备，包括2个Brooks样本Store II -20C存储系统，它们可以与样品处理和Labcyte Echo Echo plating Systems集成在一起，以提供全自动的测定板板生成。 这使NCAT为复合存储和灵活性提供了令人难以置信的能力，可以在需要的情况下运送带有定制镀板库的定制板库。 NCAT的壁内计划大量参与了NIH的Covid-19救济工作。 RSC直接参与了这些关键计划中的几项，从而在几个计划中提供了项目管理，科学和技术支持。 以下是RSC研究的一些Covid-19项目的列表： 抗病毒预防计划（APP）实验室立式：NCAT作为应用程序的一部分获得了大量资金，其中许多或责任与高吞吐量筛选，复合管理（包括反病毒库设计和复合获取），测定开发和设计以及数据共享有关。 RSC参与了所有这些活动，有助于适应整个设备，包括仪器选择，订购，设置和操作。 考虑到大量设备，这是一项大量的工作，并且在与NCATS建筑服务紧密合作时，建立了整个实验室，以在几个月内启动应用程序的工作。