Preclinical Imaging Shared Resource

临床前成像共享资源

基本信息

批准号：
10491812
负责人：
Heather Colleen Jensen Smith
金额：
$ 1.42万
依托单位：
UNIVERSITY OF NEBRASKA MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-09-05 至 2026-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10491812
关键词：
3-Dimensional 4D Imaging Anatomy Animal Behavior Animal Model Animals Atlases Basic Science Biological Biological Models Biological Phenomena Cancer Center Cancer Center Support Grant Cancer Patient Cells Cessation of life Clinical Clinical Trials Collection Complex Consultations Custom Data Data Analyses Educational workshop Electron Microscopy Equipment Etiology Experimental Designs Foundations Funding Funding Opportunities Goals Grant IACUC Image Imaging Techniques Imaging technology Individual Influentials Institution Life Magnetic Resonance Imaging Malignant Neoplasms Microscopy Modality Molecular Morphology Multimodal Imaging Mus Onset of illness Operative Surgical Procedures Optics Organ Organoids Physiological Reporter Research Research Personnel Research Proposals Research Training Resistance Resolution Resource Sharing Resources Sampling Site Specialist System Techniques Three-Dimensional Imaging Time Tissue imaging Tissues Training Training Programs Ultrasonography United States National Institutes of Health Whole Organism X-Ray Computed Tomography animal imaging animal tissue anticancer research base clinical imaging contrast imaging cost effective data integration digital experience image guided imaging capabilities imaging facilities imaging modality in vivo in vivo imaging system innovation insight instrumentation intravital imaging intravital microscopy irradiation multidimensional data outreach photoacoustic imaging preclinical imaging prospective repository response single photon emission computed tomography submicron tool translational cancer research ultrasound web site

项目摘要

PROJECT SUMMARY: PRECLINICAL IMAGING SHARED RESOURCE Understanding complex biological phenomena often governing life and death in cancer patients requires analysis and understanding at the single cell or molecular level, as well as a whole organ and/or systemic level approach/understanding. The Preclinical Imaging Center (PIC) at UNMC houses specialized imaging equipment from three complementary research cores (IVIS Optical Imaging, Ultrasonic Imaging, Multiphoton Intravital and Tissue Imaging) to allow PFBCC researchers to readily transition across imaging modalities and generate multi-level, multi-dimensional datasets. When used individually, or in combination to maximize impact and rigor, the PIC provides researchers with all the tools necessary to evaluate critical morphological, physiological, and/or cellular changes responsible for disease onset, progression, and response/resistance across a diverse set of biological samples. Within the PIC, innovative, comprehensive, and cost-effective imaging resources pertinent to a diverse collection of cancer studies are available to BCC researchers. The IVIS Optical Imaging Core (IVIS Spectrum In Vivo Imaging System) provides high throughput 2D and 3D imaging of both bioluminescent and fluorescent reporters in live animals that can be evaluated in an anatomical context using the Digital Mouse Atlas or co- registered with MRI or CT images. The Ultrasonic Imaging Core (US) houses multiple ultrasonic and photoacoustic imaging technologies allowing researchers to perform basic ultrasound, contrast imaging, Doppler, 3D and 4D imaging, and photoacoustic imaging in live animals. The Multiphoton Intravital and Tissue Imaging Core (MITI) combines intravital microscopy and multiphoton excitation/imaging to provide both high- resolution (submicron) and whole organ/tissue imaging in live animals, tissue explants, organoids, and various fixed samples in 2D and 3D. The PIC is a cancer-focused research center housed within a larger Small Animal Imaging Facility (SAIF) providing BCC researchers with access to additional small animal imaging resources including, small animal MRI, SPECT/CT, and Optical Surgical Navigation. Additional affiliated research cores (Advanced Microscopy, Electron Microscopy, Image Guided Irradiation, Animal Behavior) provide complementary resources further extending the research repertoire and data integration capabilities of our researchers. In addition to its instrumentation, by providing custom-tailored, one-on-one training, and expertise/experience in experimental design, image acquisition, and data analysis, the PIC provides cancer researchers with all the tools necessary to achieve new insights into the most daunting of research challenges. Furthermore, with the shared resources available at the PIC, BCC researchers have the exceptional opportunity to actively integrate imaging data obtained from various translational animal models using multiple imaging technologies which both parallel, and expand, clinical imaging modalities available on campus.

项目摘要：临床前成像共享资源了解通常控制癌症患者生死的复杂生物现象需要在单细胞或分子水平以及整个器官和/或系统水平上进行分析和理解方法/理解。 UNMC 的临床前成像中心 (PIC) 拥有专业成像来自三个互补研究核心（IVIS 光学成像、超声波成像、多光子活体和组织成像），使 PFBCC 研究人员能够轻松地跨成像模式和生成多层次、多维度的数据集。单独使用或组合使用以最大限度地发挥影响 PIC 为研究人员提供了评估关键形态、导致疾病发生、进展和反应/抵抗的生理和/或细胞变化跨越一组不同的生物样本。在 PIC 内，与多样化相关的创新、全面且具有成本效益的成像资源 BCC 研究人员可以获取癌症研究的集合。 IVIS 光学成像核心（IVIS Spectrum 体内成像系统）提供生物发光和荧光的高通量 2D 和 3D 成像活体动物中的报告者可以使用数字鼠标图谱或共同在解剖学背景下进行评估通过 MRI 或 CT 图像进行注册。超声波成像核心（美国）包含多个超声波和光声成像技术使研究人员能够执行基本的超声、对比成像、活体动物的多普勒、3D 和 4D 成像以及光声成像。多光子活体和组织成像核心 (MITI) 结合了活体显微镜和多光子激发/成像，提供高活体动物、组织外植体、类器官和各种器官的分辨率（亚微米）和整个器官/组织成像固定 2D 和 3D 样本。 PIC 是一家专注于癌症的研究中心，位于规模更大的小动物成像设施 (SAIF) 内为 BCC 研究人员提供额外的小动物成像资源，包括小动物 MRI、SPECT/CT 和光学手术导航。其他附属研究核心（高级显微镜、电子显微镜、图像引导照射、动物行为）进一步提供补充资源扩展我们研究人员的研究范围和数据集成能力。除了它的仪器，通过提供定制的、一对一的培训以及实验方面的专业知识/经验设计、图像采集和数据分析，PIC 为癌症研究人员提供了所有必要的工具对最艰巨的研究挑战获得新的见解。此外，通过共享资源 BCC 研究人员可以通过 PIC 获得主动整合成像数据的绝佳机会使用多种成像技术从各种转化动物模型获得，这些技术既平行又扩大校园内可用的临床成像模式。