Light-Sheet Fluorescence Microscope

光片荧光显微镜

基本信息

批准号：
10177034
负责人：
Heather Colleen Jensen Smith
金额：
$ 47.73万
依托单位：
UNIVERSITY OF NEBRASKA MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-01 至 2023-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10177034
关键词：
Acoustics Advisory Committees Anatomy Animal Model Animals Antineoplastic Agents Area Basic Science Biological Phenomena Biology Cancer Center Cells Clinical Clinical Research Clinics and Hospitals Complex Data Development Disease Elements Equipment Fluorescence Microscopy Foundations Functional disorder Funding Hospitals Hydrogels Image Imaging technology Individual Institution Light Magnetic Resonance Imaging Malignant Neoplasms Medical center Medicine Microscopic Microscopy Molecular Multimodal Imaging Nebraska Nervous System Physiology Neurosciences Optics Organ Organism Population Preclinical Drug Development Prevention Research Research Personnel Systems Integration Tissues Translational Research Ultrasonography United States National Institutes of Health Universities animal imaging animal model development base bioimaging boys data integration drug development drug discovery experience fluorescence microscope human disease innovation insight instrument multimodality nanoscale novel optical imaging polymerization programs single photon emission computed tomography tissue processing translational neuroscience translational study

项目摘要

Project Summary The biology of multicellular organisms is coordinated across multiple size scales, from the sub-nanoscale of molecules to the macroscale, to tissue-wide interconnectivity of cell populations in complex tissues, organs, and organisms. Using new, state-of-the-art light-sheet fluorescence microscopy (LSFM) and optical clearing (CLARITY) of tissues, current animal models of development, dysfunction, and disease can be appreciated with innovative insights gained only when microscopic analyses (individual cells) are observed and analyzed over macroscopic distances (intact tissues, organs, organisms). This proposal represents a shared request from various NIH-funded researchers at the University of Nebraska Medical Center (UNMC) and surrounding research institutions (Creighton University, Boys Town National Research Hospital) to obtain funding for imaging, tissue processing, and data integration equipment, the Miltenyi UltraMicroscope II, X-Clarity Tissue Hydrogel Polymerization and Clearing System, and data integration workstation not currently available in Nebraska. This type of micro- to macro-scale imaging is of paramount importance for the diverse array of animal studies conducted by cancer biologists, neuroscientists, cell biologists, physiologists, and developmental biologists at UNMC and surrounding institutions. Of the many basic, translational and clinical research centers and programs at UNMC, this proposal will principally serve three focus areas, 1) Cancer (Fred and Pamela Buffett Cancer Center), 2) Neuroscience (Center for Integrative and Translational Neuroscience), and 3) Preclinical drug development and discovery (multiple basic, translational, and clinical researchers aligned with affiliated Nebraska Medicine Hospital and clinics). The proposed instrument cluster led by Dr. Jensen Smith (19 years of biomedical imaging experience) with oversight provided by an advisory committee consisting of Directors of affiliated imaging cores, will provide a robust opportunity to support the strong user base of 17 NIH-funded researchers. We have combined the foundational elements for developing a novel, holistic/multimodal imaging pipeline at UNMC merging structural/anatomical data (MRI, SPECT/CT, Ultrasound, LSFM) with functional and molecular data (intravital optical imaging, photo-acoustics, multiphoton and advanced microscopy) to expand and elevate the rigor of ongoing studies using animal models to understand human disease. In addition to strong user support and demand, the current proposal has strong institutional, inter- and intra-institutional support. As part of a multimodal continuum of animal imaging technologies, the UltraMicroscope II and associated equipment will provide powerful perspectives of various biological phenomena thereby accelerating translational studies aimed at prevention and treatment of human diseases including, neurological function/dysfunction, cancer, and drug development/discovery.

项目概要多细胞生物的生物学在多个尺寸尺度上协调，从亚纳米尺度到分子到宏观尺度，到复杂组织、器官和细胞群的组织范围内的互连性有机体。使用最先进的新型光片荧光显微镜 (LSFM) 和光学透明技术（清晰）组织、当前动物模型的发育、功能障碍和疾病可以通过只有通过观察和分析微观分析（单个细胞）才能获得创新见解宏观距离（完整的组织、器官、生物体）。该提案代表了来自以下各方的共同请求：内布拉斯加大学医学中心 (UNMC) 及周边研究的多位 NIH 资助的研究人员机构（克赖顿大学、男孩镇国家研究医院）获得成像、组织方面的资金处理和数据集成设备，Miltenyi UltraMicroscope II，X-Clarity 组织水凝胶聚合和清除系统以及数据集成工作站目前在内布拉斯加州尚不可用。这微观到宏观成像类型对于各种动物研究至关重要由癌症生物学家、神经科学家、细胞生物学家、生理学家和发育生物学家进行 UNMC 及周边机构。许多基础、转化和临床研究中心和项目在 UNMC，该提案将主要服务于三个重点领域，1) 癌症（弗雷德和帕梅拉·巴菲特癌症中心），2）神经科学（整合和转化神经科学中心），以及3）临床前药物开发和发现（多个基础、转化和临床研究人员与附属机构保持一致）内布拉斯加州医学医院和诊所）。拟议的仪表组由 Jensen Smith 博士（19 年生物医学成像经验），并由由董事组成的咨询委员会提供监督附属成像核心，将为支持 17 个 NIH 资助的强大用户群提供强大的机会研究人员。我们结合了开发新颖的整体/多模态成像的基本要素 UNMC 的管道将结构/解剖数据（MRI、SPECT/CT、超声、LSFM）与功能和分子数据（活体光学成像、光声、多光子和先进显微镜）扩展并提高正在进行的利用动物模型了解人类疾病的研究的严谨性。除了实力雄厚用户的支持和需求，当前的提案得到了强有力的机构、机构间和机构内的支持。作为 UltraMicroscope II 和相关设备是动物成像技术多模式连续体的一部分将为各种生物现象提供强有力的视角，从而加速转化研究旨在预防和治疗人类疾病，包括神经功能/功能障碍、癌症和药物开发/发现。