PHTHALOCYANINE PHOTOSENDITIZER EFFICIENCY ON PTK2 CELLS

酞菁光敏剂对 PTK2 细胞的功效

• 批准号: 3767384
• 负责人: JOHN W OWENS
• 金额: --
• 依托单位: SOUTHERN UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3767384
• 关键词: animal tissue; chlorin; chlorophyll; cytotoxicity; epithelium; fluorescence; kidney cell; lasers; medical education; minority institution research support; neoplasm /cancer photoradiation therapy; photosensitizing agents; phthalocyanin; tissue /cell culture; trypan blue; water solubility

The aim of this project is to measure the fluorescence quantum yield, fluorescence lifetime, triplet quantum yield and triplet lifetime for fifteen photosensitizers, and correlate these measurements with the comparative in vitro photoinactivation efficiency of these fifteen photosensitizers in PTK2 rat kangaroo epithelial kidney cells. The fifteen photosensitizers are: phthalocyanine (Pc), its di- and tetrasulfonate (PcDs,PcTs); zinc metallated complexes of Pc, PcDs, PcTs; chloroaluminum phthalocyanine and its tetrasulfonate (CIAIPc, CIAIPcTs); napthalocyanine (NPc), ZnNpc, methyl pheophorbide a (Mppb); mono-L- aspartyl chlorin E6 (MACE); tin etiopurpurin (SnEt); hematoporphyrin-IX and Photofrin (PII), a concentrated form of HpD. Photophysical measurements will take place in Dimethylformamide (DMF) and photocytotoxicity studies will take place in phosphate buffered saline (PBS), where possible, or in PBS- cremophor for the chlorin derivatives. Photosensitizers will be introduced to PTK2 rat kangaroo epithelial cells for twenty-four hours, exposed to light at the appropriate wavelength from a Nd-YAG laser, and the photokill efficiency of each complex assessed utilizing the trypan blue test for cell viability. This experiment seeks to provide a model for predicting the photokill efficiency of photosensitizers based on their photophysical parameters. This study represents the first comprehensive study of the photo physical behavior and the photocytotoxicity efficiency of a wide variety of photosensitizers, including "new" and "old" classes of photosensitizers- phthalocyanine and its derivatives, chlorophyll and chlorin derivatives- and Photofrin II. The hypothesis is that the best photosensitizers are those which possess a long-lived triplet state in high quantum yield when the oxygen concentration remains constant.

该项目的目的是测量荧光量子产率， 荧光寿命，三重量子产率和三胞胎寿命 15个光敏剂，并将这些测量与 这十五的比较体外光激活效率 PTK2大鼠袋鼠上皮肾细胞中的光敏剂。 这 15个光敏剂是：邻苯烷氨酸（PC），其DI-和 四磺酸盐（PCD，PCTS）; PC，PCD，PCT的锌金属络合物； 邻苯胺及其四磺酸盐（Ciaipc，Ciaipcts）； 午睡酸（NPC），ZnnPC，甲基磷酸盐A（MPPB）；单l- 天冬氨酸E6（MACE）; Tin etiopurpurin（Snet）;血源性纤维蛋白 和photofrin（PII），浓缩形式的HPD。 光物理 测量将在二甲基甲酰胺（DMF）和 光毒性研究将在磷酸盐缓冲盐水中进行 （PBS），在可能的情况下或在PBS-CREMOPHOR中用于氯化衍生物。 光敏剂将被引入PTK2大鼠袋鼠上皮细胞 在二十四小时内，以适当的波长暴露于 ND-YAG激光器以及每个复合物的光数效率 利用锥虫蓝色测试来实现细胞活力。该实验寻求 提供一个模型来预测光数效率 光敏剂基于其光物理参数。 这项研究代表了照片物理的首次全面研究 各种各样的行为和光毒性效率 光敏剂，包括“新”和“旧”类光敏剂 - 邻苯丙氨酸及其衍生物，叶绿素和氯蛋白衍生物 - 和Photofrin II。假设是最好的光敏剂是 那些在高量子产量中具有长寿命的三胞胎状态时 氧浓度保持恒定。