EFFECTS OF CHLOROPHYLLS ON AFLATOXINB2 BIOAVAILABILITY IN HUMAN VOLUNTEERS

叶绿素对人类志愿者中黄曲霉毒素 B2 生物利用度的影响

• 批准号: 7724090
• 负责人: GEORGE S BAILEY
• 金额: $ 6.99万
• 依托单位: UNIVERSITY OF CALIF-LAWRNC LVRMR NAT LAB
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7724090
• 关键词: Aflatoxins; Behavior; Biological Availability; Body Fluids; Carcinogens; Chlorophyll; Computer Retrieval of Information on Scientific Projects Database; Dose; Food; Funding; Grant; Human; Human Volunteers; In Vitro; Ingestion; Institution; Mass Spectrum Analysis; Modeling; Range; Research; Research Personnel; Resources; Risk; Risk Reduction; Salmo trutta; Source; Testing; United States National Institutes of Health; Work; cancer chemoprevention; chlorin; chlorophyllin; chlorophyllin a; in vivo; uptake

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Much of this work targets on AFB1 risk reduction. We have used the trout model to identify chlorophyllin (CHL), a food-grade derivative of chlorophyll (Chl), as a potent blocking agent against AFB1. To pursue the promise of chlorophylls and derivatives as blocking agents, we will compare the efficacy of chlorophyllin (CHL) and natural chlorophylls (Chl) in cancer chemoprevention and humans. Both agents exert antimutagenesis behavior in vitro against a wide range of carcinogens. Our guiding hypothesis is that chlorins protect in vivo primarily via reduction of carcinogen uptake and bioavailability. We propose to test our central hypothesis in humans using 14C-AFB1. Accelerated Mass Spectrometry (AMS) of body fluids following ingestion of 14C-AFB1 in the nCi/ng dose range should allow us to examine CHL and Chl effects on aflatoxin bioavailability with adequate sensitivity and negligible risk.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 这项工作的大部分目标是降低 AFB1 风险。我们使用鳟鱼模型来鉴定叶绿素 (CHL)，一种叶绿素 (Chl) 的食品级衍生物，作为 AFB1 的有效阻断剂。为了追求叶绿素及其衍生物作为阻断剂的前景，我们将比较叶绿素 (CHL) 和天然叶绿素 (Chl) 在癌症化学预防和人类中的功效。这两种药物在体外都对多种致癌物发挥抗诱变作用。我们的指导性假设是二氢卟酚主要通过减少致癌物的吸收和生物利用度来发挥体内保护作用。我们建议使用 14C-AFB1 在人类身上测试我们的中心假设。摄入 nCi/ng 剂量范围内的 14C-AFB1 后，对体液进行加速质谱 (AMS) 分析，可以让我们以足够的灵敏度和可忽略的风险来检查 CHL 和 Chl 对黄曲霉毒素生物利用度的影响。