PHTHALOCYANINE PHOTOSENSITIZER ON PTK2 RAT KANGAROO EPITHELIAL KIDNEY CELLS

酞菁光敏剂对 PTK2 大鼠袋鼠肾上皮细胞的影响

• 批准号: 6122955
• 负责人: JOHN W OWENS
• 金额: $ 9.04万
• 依托单位: SOUTHERN UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-30 至 2000-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6122955
• 关键词: animal tissue; biological products; drug /agent; human tissue; minority institution research support

The aim of this project is to measure the fluorescence quantum yields, fluorescence lifetime, triplet quantum yields an triplet lifetime for fifteen photosensitizers, and correlate these measurements with the comparative in vitro photoinactivation efficiency of these fifteen photosensitizers on PTK2 kangaroo rat epithelial kidney cells. The fifteen photosensitizers are: phthalocyanine (PC), its di and tetrasulfonate (PcDs, PcTs) derivatives, zinc metallated complex of Pc, PcDs, and PcTs; chloroaluminum phthalocyanine and its tetrasulfonate (CIAIPc, CIAIPcTs); naphthalocyanine (Npc), its zinc metallated complex (ZnNPc); methyl pheophorbide a (Mppb); mono-L-aspartyl chlorin e6 (MACE); tin etiopurpurine (SnEt); hematoporphyrin-IX (Hp), and Photofrin (Pll), a concentrated form of HpD. Photophysical measurements will take place in phosphate buffered saline (PBS) where possible, or in PBS-Cremophor for the chlorin derivatives. Photosensitizers will be introduced to PTK2 cells for twenty-four hours, exposed to light at the appropriate wavelength from a Nd-YAG laser, and the photokill efficiency of each complex assessed utilizing the trypan blue test for cell viability. This experiment seeks to provide a model for predicting the photokill efficiency of photosensitizers based on their photophysical properties.

该项目的目的是测量荧光量子 产量，荧光寿命，三重量子量子产生三重态 15个光敏剂的寿命，并将其关联 比较体外光激活的测量 PTK2袋鼠老鼠这15个光敏剂的效率 上皮肾细胞。 15个光敏剂是： 邻苯丙氨酸（PC），其DI和四磺酸盐（PCD，PCTS） 衍生物，PC，PCD和PCT的锌金属复合物； 氯氨基苯胺及其四磺酸盐（CIAIPC， ciaipcts）;萘丙氨酸（NPC），其锌金属复合物 （znnpc）;甲基凤梨A（MPPB）；单叶冬叶氯蛋白E6 （锤）; Tin etiopurpurine（Snet）;血磷脂-ix（HP）和 Photofrin（PLL），浓缩形式的HPD。 光物理 测量将在磷酸盐缓冲盐水（PBS）中进行 可能的，或用于氯化衍生物的PBS-侵略者。 光敏剂将用于二十四 小时，从ND-yag处于适当的波长处暴露于光线 激光以及每个复合物的光数效率利用 用于细胞活力的锥类蓝色测试。 这个实验试图 提供了预测光数效率的模型 光敏剂基于其光物理特性。