Basic Science Core - Imaging

基础科学核心 - 成像

基本信息

批准号：
10431470
负责人：
Deepak Kaushal
金额：
$ 19.99万
依托单位：
TEXAS BIOMEDICAL RESEARCH INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-03-10 至 2027-02-28
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10431470
关键词：
Address Animal Model Animals Basic Science Biology Biomedical Research Caring Cell Separation Cells Cellular Immunology Cities Clinical Clinical Management Clinical Services Collaborations Communities Complement Computational algorithm Computer Analysis Confocal Microscopy Containment Data Disadvantaged Discipline Education Educational Curriculum Emission-Computed Tomography Equipment Evaluation Flow Cytometry Fluorescent in Situ Hybridization Fluorochrome Freezing Funding Funding Agency Genomics Goals Health Professional Image Image Analysis Immunofluorescence Immunologic Inpatients Institutes Knowledge Label Laboratories Lasers Leadership Learning Light Magnetic Resonance Imaging Mexico Microscopy Molecular Optics Outpatients Paraffin Participant Positron-Emission Tomography Primates Publishing RNA Recording of previous events Research Research Institute Research Personnel Resources Robotics Rodent Science Scientist Services Sorting - Cell Movement Structure System Techniques Testing Texas Thick Tissues Training Training Programs Tuberculosis United States National Institutes of Health Work X-Ray Computed Tomography animal safety base biocontainment facility cohort design experience experimental study high dimensionality imaging capabilities imaging modality imaging platform imaging study in vivo imaging system live cell imaging microscopic imaging multidisciplinary next generation optical imaging peer prevent programs prospective skills tool whole animal imaging

项目摘要

TEXAS BIOMED IN-TRAC Research Imaging Core ABSTRACT The Research Imaging Core will leverage key strengths of Texas Biomed, namely a strong cohort of NIH- funded TB investigators with a history of collaboration and with experience in different imaging platforms. IN- TRAC participants will benefit from access to unique NHP resources in the Southwest National Primate Research Center (SNPRC) and its cutting edge imaging capabilities. The Research Imaging Core will also establish an education curriculum to introduce IN-TRAC participants to a variety of cell-based and whole animal imaging platforms. AIM 1: Provide didactic and hands-on training program in confocal microscopy and optical imaging. IN-TRAC participants will learn multi-label immunofluorescence or fluorescence in-situ hybridization (FISH) confocal microscopy, which allows for 3- or 4-D evaluation of tissues and cells with multiple fluorochrome labels. AIM 2. Provide didactic and hands-on training in cutting-edge cellular immunology techniques requiring imaging support or computational analysis. The Texas Biomed Biology, Microscopy and Molecular Cores provide high-dimensional, multi-parameter flow cytometry and analysis as well as BSL3 sorting capabilities. Established TB researchers will provide training live cell imaging (Cytation 5) and scRNAseq (10x Genomics) in BSL3. AIM 3: Provide didactic and hands-on training in live imaging using PET/CT and IVIS systems. Training in whole-body live Positron Emission Tomography (PET) and X-ray Computed Tomography (CT) imaging of NHP and in-vivo imaging system (IVIS) of rodents within the BSL3. IN-TRAC participants will extern at the NHP MRI facilities at UTHSCA to learn the advantages and limitations of this imaging platform. At the completion of the Research Imaging Core, participants will be able to effectively interpret published data that use imaging modalities for NHP, rodents, tissues, cells and RNA; design experiments to test their own hypothesis; and participate in TB research that uses the appropriate animal model.

德克萨斯州 BIOMED IN-TRAC 研究成像核心摘要研究成像核心将利用 Texas Biomed 的关键优势，即强大的 NIH 团队资助具有合作历史和不同成像平台经验的结核病研究人员。在- TRAC 参与者将受益于西南国家灵长类研究中心独特的 NHP 资源中心 (SNPRC) 及其尖端成像能力。研究成像核心还将建立一个向 IN-TRAC 参与者介绍各种基于细胞和整体动物成像的教育课程平台。目标 1：提供共焦显微镜和光学方面的教学和实践培训计划成像。 IN-TRAC 参与者将学习多标记免疫荧光或荧光原位杂交 (FISH) 共焦显微镜，可使用多种荧光染料对组织和细胞进行 3 或 4 维评估标签。目标 2. 提供尖端细胞免疫学技术的教学和实践培训需要成像支持或计算分析。德克萨斯生物医学生物学、显微镜学和分子学核心提供高维、多参数流式细胞术和分析以及 BSL3 分选能力。成熟的结核病研究人员将提供活细胞成像 (Cytation 5) 和 scRNAseq (10x BSL3 中的基因组学）。目标 3：使用 PET/CT 和 IVIS 提供实时成像的教学和实践培训系统。全身实时正电子发射断层扫描 (PET) 和 X 射线计算机断层扫描培训 BSL3 内 NHP 的 (CT) 成像和啮齿动物体内成像系统 (IVIS)。 IN-TRAC 参与者将 UTHSCA 的 NHP MRI 设施的实习生，了解该成像平台的优点和局限性。在研究成像核心完成后，参与者将能够有效地解释已发布的数据使用 NHP、啮齿动物、组织、细胞和 RNA 的成像方式；设计实验来测试自己假设;并参与使用适当动物模型的结核病研究。