CELLULAR/MOLECULAR ANALYSIS OF SHORT & LONG TERM MEMORY

短路的细胞/分子分析

• 批准号: 2245092
• 负责人: TERRY J CROW
• 金额: $ 20.08万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-03-01 至 2000-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2245092
• 关键词: Hermissenda; afferent nerve; alternatives to animals in research; behavior; behavior prediction; behavioral medicine; biological models; dopamine; ear hair cell; electrophysiology; fluorescent dye /probe; interneurons; invertebrate locomotion; model design /development; motor neurons; neuropeptides; neuropharmacology; neurophysiology; neurotransmitter receptor; neurotransmitters; photobiology; saltwater environment; serotonin; single cell analysis; synapses; videotape /videodisc; visual threshold

A major goal of neurobiological research is the understanding of the underlying organization of neurotransmitter and receptor systems related to the function of the nervous system. One approach to this problem is to study the synaptic physiology of a well-documented neural circuit that subserves a specific behavior. This approach has been successfully applied to an analysis of the relative simple nervous systems in many invertebrates. One preparation that has been useful in a cellular analysis of behavior is the marine mollusk Hermissenda. The neural circuit underlying a well-defined and quantified example of behavior will be studied using a combination of cellular neurophysiological and pharmacological techniques. Using intracellular recording and stimulation techniques putative motor neurons and interneurons that receive synaptic input from the visual system will be identified and the response to light classified. The relative contribution of identified pedal neurons and interneurons to the network underlying phototactic behavior will be studied in intact animals after eliminating specific neurons by the photoinactivation technique. Changes in behavior will be assessed following the selective elimination of specific neurons in the neuronal circuit including specific photoreceptor types, interneurons, and putative motor neurons. The neurotransmitters mediating the synaptic interactions among the neurons of this network will be examined using pharmacological and electrophysiological techniques. The role of biogenic amines such as dopamine and serotonin in modulating the activity of primary sensory neurons and putative motor neurons will be studied in the isolated nervous system. Since peptides may occur together with other neurotransmitters, the effects of various peptides on the photoresponse will be examined. An understanding of the neurotransmitter and receptor systems in a relatively simple neural network and its modulation by biogenic amines and peptides will provide a useful model system for studies of affective disorders in more complex mammalian nervous systems.

神经生物学研究的主要目标是对 神经递质和受体系统的基础组织 神经系统的功能。 解决这个问题的一种方法是 研究有据可查的神经回路的突触生理学 实行特定的行为。 这种方法已成功应用 分析许多人中的相对简单神经系统 无脊椎动物。 一种在细胞分析中有用的制剂 行为是海洋软体动物hermissenda。 神经回路 基本的定义明确和量化的行为示例将是 使用细胞神经生理学和 药理技术。 使用细胞内记录和刺激 接受突触的技术假定的运动神经元和中间神经元 将确定来自视觉系统的输入，并对光的响应 分类。 已识别的踏板神经元的相对贡献和 将研究光电行为的基础网络中的中间神经元 在完整动物中消除了特定神经元之后 光活化技术。 行为的变化将评估 选择性消除神经元中的特定神经元 电路包括特定的光感受器类型，中间神经元和推定的电路 运动神经元。 介导突触相互作用的神经递质 在该网络的神经元中，将使用药理学检查 和电生理技术。 生物胺的作用，例如 多巴胺和5-羟色胺调节主要感觉的活性 神经元和推定的运动神经元将在孤立的神经中进行研究 系统。 由于肽可能与其他神经递质一起发生，因此 将检查各种肽对光响应的影响。 一个 了解相对较大的神经递质和受体系统 简单的神经网络及其对生物胺和肽的调节 将为研究情感障碍的研究提供有用的模型系统 更复杂的哺乳动物神经系统。