Computational model of cellular plasticity

细胞可塑性的计算模型

• 批准号: 6318432
• 负责人: TERRY J CROW
• 金额: $ 19.39万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-01 至 2001-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6318432
• 关键词: Hermissenda; action potentials; alternatives to animals in research; behavioral /social science research tag; biophysics; computational neuroscience; conditioning; mathematical model; model design /development; molecular psychobiology; neural information processing; neural plasticity; visual pathways; visual photoreceptor; visual stimulus; voltage /patch clamp

The primary goal of this research project is to develop a mathematical model of the light-elicited generator potential and spike generation in the photoreceptors of Hermissenda. Paviovian conditioning of Hermissenda results in the modification of several K+ conductances in identified cells of the pathway supporting the conditioned stimulus (CS). The preparation is especially well suited for a biophysical analysis since the voltage- and light-dependent conductances of identified photoreceptors have been characterized in some detail with voltage-clamp techniques. In addition, conventional cellular neurophysiological techniques have identified several neural correlates of conditioning in the identified photoreceptors of the CS pathway. These recent results provide an opportunity to directly relate biophysical modifications in identified cells to neural correlates of conditioning that are expressed by changes in excitability and synaptic strength. The application of computer simulations of the photoreceptors would be used to help elucidate the role of diverse biophysical changes to the modification of excitability and synaptic strength in identified cells of conditioned animals. The proposal consists of the following four specific aims: 1) Use the voltage and light-dependent conductances of the photoreceptors derived from voltage-clamp studies to develop a quantitative model of the light-elicited generator potentials; 2) Use the quantitative model to examine the correspondence between modifications in various membrane conductances produced by conditioning and the modification of generator potentials and spike frequency predicted by the conductance changes; 3) Use the quantitative model to identify conductance changes that may contribute to the modification of generator potentials and spike frequency produced by conditioning; and 4) Develop a network model of CS pathway activity that incorporates known changes in excitability in identified cells and changes synaptic strength at identified synaptic connections. These duties will provide important insights into our understanding of the physiology of learning and memory.

该研究项目的主要目标是开发 Hermissenda 光感受器中光引发发电机电位和尖峰生成的数学模型。 Hermissenda 的巴维奥条件作用导致支持条件刺激 (CS) 通路的已识别细胞中多个 K+ 电导的改变。准备工作是 特别适合生物物理分析，因为已使用电压钳技术对所识别的光感受器的电压和光依赖性电导进行了一些详细的表征。此外，传统的细胞神经生理学技术已经确定了在已识别的光感受器中调节的几个神经相关性。 CS途径。这些最近的结果提供了一个机会，将已识别细胞中的生物物理修饰与通过兴奋性和突触强度的变化表达的调节的神经相关性直接联系起来。光感受器的计算机模拟的应用将有助于阐明各种生物物理变化对光感受器的作用。 调节动物的已识别细胞的兴奋性和突触强度的改变。该提案包括以下四个具体目标：1）利用电压钳研究得出的光感受器的电压和光依赖性电导来开发一种 光引发发电机电势的定量模型； 2）使用定量模型检查调节产生的各种膜电导的变化与电导变化预测的发生器电位和尖峰频率的变化之间的对应关系； 3）利用定量模型进行识别 电导变化可能有助于改变调节产生的发生器电位和尖峰频率； 4) 开发 CS 通路活动的网络模型，其中包含已识别细胞中已知的兴奋性变化，并改变已识别突触连接处的突触强度。这些职责将为我们提供重要的见解 了解学习和记忆的生理学。