CONTROL OF THE START OF THE BUDDING YEAST CELL CYCLE

控制出芽酵母细胞周期的开始

• 批准号: 2184628
• 负责人: FREDERICK R. CROSS
• 金额: $ 26.74万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-05-01 至 1997-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2184628
• 关键词: Saccharomyces cerevisiae; biochemical evolution; cell cycle; fungal genetics; fusion gene; gene expression; gene mutation; genetic mapping; genetic regulation; genetic transcription; hormone regulation /control mechanism; human genetic material tag; immunoprecipitation; laboratory rabbit; molecular cloning; nucleic acid sequence; posttranscriptional RNA processing; protein structure function; site directed mutagenesis; synchronous cell division; western blottings

The focus of our work is the regulation of the G1/S transition in the budding yeast, S. cerevisiae. Previous work in several labs, including ours, has established the CLN1, CLN2, and CLN3 genes of yeast as critical in regulation of this transition, both by external factors (hormones, nutrients) and internal factors (cell size). The homology of these genes with cyclins, and their genetic and biochemical interactions with the product of the cdc2 homolog in this organism, CDC28, combined with the apparent specificity of their action to the G1/S transition (as opposed to the G2/M transition for previously described cyclins) suggests the idea that the CLN proteins may be 'G1 cyclins'. Since the G1/S transition is a primary point of regulation in many organisms, including humans, and since the regulation of this transition is grossly abnormal in cancer, it is important to determine the mechanism of action and regulation of CLN genes, as well as to determine the universality through evolution of the system. These are the issues addressed in this application.

我们工作的重点是对G1/s过渡的调节 酿酒酵母的芽酵母。以前在多个实验室中的工作，包括 我们已经确立了酵母的CLN1，CLN2和CLN3基因 在调节这种过渡时，无论是外部因素（激素， 营养）和内部因素（细胞大小）。 这些基因的同源性 与细胞周期蛋白及其遗传和生化相互作用 该生物中Cdc2同源物的产物Cdc28与 他们对G1/S转变的行动明显特异性（而不是 对先前描述的细胞周期蛋白的G2/M转变）表明 CLN蛋白可能是“ G1细胞周期蛋白”的想法。 由于G1/s 过渡是许多生物的主要调节点，包括 人类，并且由于这种过渡的调节非常异常 在癌症中，确定作用机理和 CLN基因的调节，并通过 系统的演变。 这些是其中解决的问题 应用。