STUDIES OF YEAST CDC14

酵母的研究 CDC14

• 批准号: 8169122
• 负责人: FREDERICK R. CROSS
• 金额: $ 0.12万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169122
• 关键词: Antibodies; Cell Cycle; Cell Cycle Progression; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Housing; Institution; Methods; Phosphoric Monoester Hydrolases; Proteins; Research; Research Personnel; Resources; Source; United States National Institutes of Health; Yeasts; magnetic beads; mutant

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We use our newly developed method for the isolation of GFP-tagged proteins to study the cell-cycle dependent interactions of the phosphatase, Cdc14,required for cell cycle progression. Large scale yeast cultures were grown for two mutants of Cdc14, one localized at the spindle pole and the other with cytoplasmic localization. We have performed isolations using magnetic beads coated with our in-house developed anti-GFP antibodies and identified Cdc14-interacting partners specific for each strain.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 我们使用新开发的方法来分离GFP标记的蛋白质来研究细胞周期进程所需的磷酸酶CDC14的细胞周期依赖性相互作用。大规模的酵母培养物是针对Cdc14的两个突变体生长的，一种是位于纺锤极，另一个位于细胞质定位。 我们使用内部开发的抗GFP抗体的磁珠进行了分离，并确定了针对每个菌株的CDC14相互作用伴侣。