IMAGE-READY FIBER-OPTIC SENSORS FOR BIOMEDICAL ANALYSIS

用于生物医学分析的图像就绪光纤传感器

• 批准号: 2185596
• 负责人: DAVID R. WALT
• 金额: $ 14.33万
• 依托单位: TUFTS UNIVERSITY MEDFORD
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-01 至 1997-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2185596
• 关键词: biomedical equipment development; charge coupled device camera; fiber optic microscopy; histochemistry /cytochemistry; monitoring device

The work described in this application is aimed at proving the utility of sensors prepared with imaging optical fibers. This project aims to prepare fiber optic sensors for monitoring numerous parameters simultaneously and potentially invasively. The approach offers major advantages over other sensing methods which all employ a distinct sensor for each parameter measured. We plan to apply the method to simultaneous imaging and chemical monitoring. This approach should allow positional and morphological information obtained by imaging through the fibers to be combined with simultaneous chemical analysis of the area of interest. The power of this combined imaging and measuring approach will be demonstrated by applications in both clinical and basic research. Measurements of local values of pH and 02 in precisely localized regions adjacent to living cells and tissues will be accomplished with kidney cell cultures as well as whole organ preparations. The technique involves coupling polymer layers to a coherent imaging fiber bundle of less than several hundred micromoles in diameter. Precise positioning of individual sensing elements on the fiber's distal tip is accomplished via photopolymerization. The signals emanating from the resulting sensor array are detected by means of a Charge Coupled Device (CCD) camera. The combination of imaging fibers and a CCD camera enable spatial information to be preserved. The addition of spatial information to the spectral information in the individual sensing elements provides technical expediency as well as opens the doors for a variety of scientifically important experiments and clinical measurements. This work aims to establish chemical coating techniques for these unique sensors that can be generalized to a wide range of important analytes. In addition the proposal seeks to develop the requisite supporting instrumentation to enable these sensors to be used for biomedical analysis and chemical microscopy.

本申请中描述的工作旨在证明 使用成像光纤制备的传感器。该项目旨在准备 光纤传感器，用于同时监视许多参数，并且 潜在的侵入性。该方法比其他方法具有重大优势 感应方法，所有参数都采用独特的传感器 测量。我们计划将方法应用于同时成像和化学 监视。这种方法应允许位置和形态学 通过通过纤维成像与以要结合的纤维获得的信息 同时对感兴趣领域进行化学分析。这个力量 结合成像和测量方法将通过 在临床和基础研究中的应用。本地的测量 精确本地化区域的pH值和02的值 细胞和组织也将通过肾细胞培养物完成 作为整个器官制剂。 该技术涉及将聚合物层耦合到连贯的成像纤维 直径少于几百微孔的束。精确的 纤维远端的单个感应元素的定位是 通过光聚合完成。从 通过电荷耦合设备检测到的传感器阵列 （CCD）相机。成像纤维和CCD相机的组合启用 要保留的空间信息。 添加空间信息 在单个感应元素中的光谱信息提供 技术权宜以及为各种各样的大门打开 科学重要的实验和临床测量。这项工作 旨在为这些独特传感器建立化学涂料技术 可以推广到广泛的重要分析物。此外 该提案旨在开发必要的支持工具 使这些传感器可用于生物医学分析和化学 显微镜。