ShEEP Request for MESO SECTOR S 600MM Ultra-Sensitive Plate Imager

ShEEP 请求 MESO SECTOR S 600MM 超灵敏板成像仪

• 批准号: 10741205
• 负责人: NICHOLAS J WEBSTER
• 金额: --
• 依托单位: VA SAN DIEGO HEALTHCARE SYSTEM
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-03-01 至 2023-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10741205
• 关键词: Acute Renal Failure with Renal Papillary Necrosis; Affect; Alcohol abuse; Alcoholic Liver Diseases; Alzheimer' s Disease; Amyloid beta-Protein; Anesthesiology; Animals; Anti-Inflammatory Agents; Award; Bacteria; Behavioral Research; Binding Proteins; Biological Assay; Biological Markers; Bipolar Disorder; Bone Diseases; CCL2 gene; CXCL1 gene; Cardiology; Cardiovascular Diseases; Caregivers; Cell Differentiation process; Cells; Chronic Kidney Failure; Clinical; Communities; Complex; Computer software; Critical Care; Crohn' s disease; Dedications; Degenerative polyarthritis; Development; Diabetes Mellitus; Disease; Endocrinology; Environment; FGF2 gene; Funding; G-Protein-Coupled Receptors; Gastroenterology; Glucagon; Glutathione S-Transferase; Head; Healthcare; Healthcare Systems; Heart Injuries; Human; Hypersensitivity; Hypertension; IL17 gene; IL18 gene; Immune; Immune System Diseases; Immunology; Industrialization; Infection; Inflammation; Inflammatory; Inflammatory Bowel Diseases; Injury; Injury to Kidney; Innate Immune Response; Insulin; Interleukin-10; Interleukin-13; Interleukin-15; Interleukin-4; Interleukin-6; Interleukin-8; Interleukins; Intestines; LIF gene; Leptin; Lung; Malignant Neoplasms; Measures; Medical center; Metabolic; Methamphetamine; Mind; Mission; Mus; Natural Immunity; Nephrology; Nerve Degeneration; Neurobiology; Neurology; Noise; Obesity; Osteogenesis; Patients; Phosphoproteins; Plasma; Pre-Clinical Model; Primary Lateral Sclerosis; Principal Investigator; Proliferating; Protein Secretion; Proteins; Psychiatry; RANTES; Rattus; Reagent; Rehabilitation therapy; Research; Research Activity; Research Personnel; Rheumatology; Sampling; Sheep; Signal Transduction; Signaling Protein; Site; Speed; Stratification; TIE-2 Receptor; TNF gene; Terminal Ileitis; Testing; Therapeutic; Tissues; Toxicology; Translational Research; Traumatic Brain Injury; Tubulin; Urology; Vascular Endothelial Growth Factor Receptor-1; Vascular Endothelial Growth Factors; Veterans; Viral Pathogenesis; adaptive immune response; adaptive immunity; addiction; angiogenesis; arginase; beta secretase; calbindin; cell growth; cell type; charge coupled device camera; chemokine; clinical care; cytokine; design; fatty acid-binding proteins; hereditary angioneurotic edema; imager; imaging system; improved; instrument; interleukin-21; liver injury; mineralization; multiplex assay; pre-clinical; pre-clinical research; receptor; sulfated glycoprotein 2; systems research; tumorigenesis

Project Summary The MESO SECTOR S 600MM Ultra-Sensitive Plate Imager (with Methodical Mind software) is the flagship instrument of MSD. Its high-speed, low noise CCD camera provides high sensitivity, wide dynamic range, and rapid read times. This instrument equipped with 96 well and 364 well capacity delivers the industrial-grade throughput and reliability required in the most demanding research environments. This instrument can be used for the following applications: (i) metabolic research, (ii) immunology/inflammation research, (iii) bone disorders, (iv) cardiovascular diseases, (v) neurobiology/neurodegeneration research, and toxicology studies that are associated with cardiac injury, kidney injury, and liver injury. Cytokines and chemokines are small signaling proteins secreted primarily by immune cells. Their main function is to coordinate cell growth, proliferation, and differentiation. Cytokines and their receptors participate in a diverse array of functions including innate and adaptive immunity, inflammation, immune cell differentiation, angiogenesis, tumorigenesis, development, neurobiology, and viral pathogenesis. Dysregulation of cytokine expression is a cause of immunological and inflammatory diseases, as well as other disease conditions. Many different cell types secrete chemokines, most often to attract immune cells to the site of infection or injury during innate and adaptive immune responses. The capability to measure cytokines/chemokines in small tissue or plasma samples will significantly and positively impact many VA-sponsored projects in basic, translational, and preclinical biomedical/behavioral research relevant to Veteran’s healthcare currently conducted at the VA San Diego Healthcare System (VASDHS). Cytokines/chemokines are affected in several disease states including hypertension, diabetes, obesity, cancer, osteoarthritis, addiction, traumatic disorders, bipolar disorders, inflammatory bowel diseases, alcohol abuse, and chronic kidney diseases. We are a group of 10 currently VA funded and 5 previously VA funded investigators with VA Merit and CDA Awards who will directly benefit from acquisition of VASDHS does not have the MESO SECTOR S 600MM Ultra-Sensitive Plate Imager. Our projects encompass a wide range of fields and research, including nephrology/hypertension, endocrinology, neurology, anesthesiology, pulmonary and critical care, gastroenterology, rheumatology, immunology, cardiology, urology, and psychiatry. The acquisition of the VASDHS does not have the MESO SECTOR S 600MM Ultra-Sensitive Plate Imager will not only impact basic pre-clinical research at VASDHS, but also is expected to have a strong and positive effect through improvement of patient clinical care and supporting the VA healthcare mission.

项目概要 MESO SECTOR S 600MM 超灵敏板成像仪（配有 Methodical Mind 软件）是旗舰产品 MSD 仪器的高速、低噪声 CCD 相机提供高灵敏度、宽动态范围和 该仪器配备 96 孔和 364 孔容量，提供工业级的读取时间。 该仪器可用于最苛刻的研究环境所需的吞吐量和可靠性。 适用于以下应用：(i) 代谢研究，(ii) 免疫学/炎症研究，(iii) 骨骼疾病， (iv) 心血管疾病，(v) 神经生物学/神经变性研究和毒理学研究 与心脏损伤、肾损伤和肝损伤相关的细胞因子和趋化因子是小信号传导。 主要由免疫细胞分泌的蛋白质，其主要功能是协调细胞生长、增殖和免疫功能。 细胞因子及其受体参与多种功能，包括先天和分化。 适应性免疫、炎症、免疫细胞分化、血管生成、肿瘤发生、发育、 细胞因子表达失调是免疫学和病毒发病机制的一个原因。 炎症性疾病以及其他疾病状况许多不同的细胞类型会分泌趋化因子。 通常在先天性和适应性免疫反应期间将免疫细胞吸引到感染或损伤部位。 测量小组织或血浆样品中的细胞因子/趋化因子的能力将显着且 对许多 VA 资助的基础、转化和临床前生物医学/行为项目产生积极影响 目前在 VA 圣地亚哥医疗系统进行的与退伍军人医疗保健相关的研究 (VASDHS) 细胞因子/趋化因子在多种疾病状态下受到影响，包括高血压、糖尿病、 肥胖、癌症、骨关节炎、成瘾、创伤性疾病、双向情感障碍、炎症性肠病、 我们目前由 VA 资助的 10 人以及之前 VA 资助的 5 人组成。 获得 VA 优异奖和 CDA 奖的资助研究人员将直接从收购 VASDHS 中受益 没有 MESO SECTOR S 600MM 超灵敏板成像仪 我们的项目涵盖广泛。 领域和研究，包括肾病学/高血压、内分泌学、神经病学、麻醉学、 肺部和重症监护、胃肠病学、风湿病学、免疫学、心脏病学、泌尿学和精神病学。 收购 VASDHS 不具备 MESO SECTOR S 600MM 超灵敏平板成像仪 不仅影响 VASDHS 的基础临床前研究，而且预计将产生强大而积极的影响 通过改善患者临床护理和支持 VA 医疗保健使命。