ORGANIZATION OF YEAST RIBOSOMAL PROTEIN GENES

酵母核糖体蛋白基因的组织

• 批准号: 2174115
• 负责人: MICHAEL ROSBASH
• 金额: $ 22.46万
• 依托单位: BRANDEIS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-02-01 至 1998-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2174115
• 关键词: DNA footprinting; RNA splicing; chemical structure function; fungal genetics; gene expression; genetic library; immunoprecipitation; molecular cloning; mutant; nucleic acid sequence; polymerase chain reaction; precursor mRNA; protein biosynthesis; protein engineering; protein sequence; protein structure function; radiotracer; ribosomal proteins; small nuclear RNA; spliceosomes; tissue /cell culture; transcription factor; yeasts

Pre-mRNA splicing is an almost ubiquitous feature of gene expression. Introns must be properly removed for protein synthesis to take place correctly. Introns are recognized by trans-acting splicing factors during the early steps of spliceosome assembly, and this recognition ensures that pre-mRNA does not escape to the cytoplasm before intron removal. Splice sites are also identified at this early splicing step, and their identification contributes to the fidelity of splicing and the choice of splice site partners, namely, which exons are chosen to be ligated together. Many diseases are caused by difficulties with splice site selection, and regulation of viral gene expression during infection by HIV is dependent upon aspects of this pre-mRNA recognition and transport system. In yeast, mammalian, and viral systems, Ul snRNP is an important factor in these early splice site identification events. The nature of the yeast Ul snRNP-pre-mRNA interaction will be investigated in vitro, and the genetic advantages of yeast will be applied to the study of this snRNP and its constituents. Major foci are the structure and function of several Ul snRNP proteins, including the well studied UIA protein. We are also interested in testing the hypothesis that pre-mRNA structure plays an important role in these early recognition-identification events. pre-mRNA will be structure probed in vivo, and the effects of model structures on splicing assessed. Genetic methods will be applied to identify proteins that might modulate pre-mRNA structure or splice site selection.

前mRNA剪接是基因表达的几乎普遍存在的特征。 必须正确去除内含子以进行蛋白质合成 正确。内含子在跨作用剪接因子中识别 剪接体大会的早期步骤，这种认可确保了 前MRNA在内含子去除前不会逃到细胞质。 拼接 在此早期的拼接步骤中也确定了站点，他们 识别有助于剪接的保真度和选择 剪接网站合作伙伴，即选择外显子 一起。许多疾病是由剪接网站困难引起的 HIV感染期间病毒基因表达的选择和调节 取决于此前MRNA识别和运输的各个方面 系统。在酵母，哺乳动物和病毒系统中，UL SNRNP是重要的 这些早期剪接站点识别事件的因素。的本质 酵母UL SNRNP-PRE-MRNA相互作用将在体外研究，并将 酵母的遗传优势将应用于该SNRNP的研究和 它的成分。主要焦点是几个UL的结构和功能 SNRNP蛋白，包括研究良好的UIA蛋白。我们也是 有兴趣测试前MRNA结构的假设 在这些早期识别识别事件中的重要作用。前mRNA 将在体内探测结构，模型结构对 剪接评估。遗传方法将用于鉴定蛋白质 这可能会调节前MRNA结构或剪接位点选择。