ANTITUMOR COMPOUNDS FROM MARINE INVERTEBRATES

来自海洋无脊椎动物的抗肿瘤化合物

• 批准号: 3088250
• 负责人: MELEDATH E GOVINDAN
• 金额: $ 8.47万
• 依托单位: UNIVERSITY OF THE VIRGIN ISLANDS
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-05 至 1997-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3088250
• 关键词: Anthozoa; DNA damage; Porifera; Saccharomyces cerevisiae; Tunicata; alternatives to animals in research; animal extract; antineoplastics; bioassay; chemical structure; cytotoxicity; drug screening /evaluation; gel filtration chromatography; high performance liquid chromatography; interferometry; mass spectrometry; nuclear magnetic resonance spectroscopy; toxicology

Naturally occurring organic compounds have found numerous applications in medicine for centuries. Many drugs that are in clinical use were originally discovered as constituents of plants and herbs that were used to cure illnesses. Though the traditional natural products chemistry was limited to the isolation and characterization of secondary metabolites from terrestrial plants and animals, recent years have seen many scientists exploring the marine flora and fauna in search of novel secondary metabolites with medicinal properties. Compounds with antibacterial, antifungal, antitumor, antiviral, analgesic, and other properties have been discovered. This proposal is for a research training program involving isolation and characterization antitumor compounds from marine invertebrates. A mechanism-based bioassay involving DNA repair deficient yeast strains will be used as the preliminary screen for antitumor activity as well as to guide the separation of active compounds. Structures of active compounds will be determined using spectroscopic methods. The research will be conducted in collaboration with the sponsor, Dr. David G. I. Kingston of the Virginia Polytechnic Institute and State University. The applicant has been a faculty member at the University of the Virgin Islands, a Historically Black University in the U. S. Virgin Islands and recently he has been recommended for tenure. The applicant has been involved in research with undergraduate minority students throughout his career at UVI. The proposed research training will provide advanced training for the applicant in various bioassay procedures and state-of-the-art techniques employed in structure determination of natural products, which, in turn will allow him to continue a truly independent research program in this field at UVI.

天然存在的有机化合物在以下领域有着广泛的应用： 几个世纪以来的医学。许多临床使用的药物 最初发现为所使用的植物和草药的成分 来治愈疾病。尽管传统的天然产物化学 仅限于次生代谢物的分离和表征 近年来，从陆生植物和动物中发现了许多 科学家探索海洋动植物群以寻找新奇 具有药用特性的次生代谢物。化合物与 抗菌、抗真菌、抗肿瘤、抗病毒、镇痛等 属性已被发现。 该提案适用于一项涉及隔离和治疗的研究培训计划 表征海洋无脊椎动物的抗肿瘤化合物。一个 涉及 DNA 修复缺陷酵母菌株的基于机制的生物测定将 用作抗肿瘤活性的初步筛选以及 指导活性化合物的分离。活性化合物的结构 将使用光谱方法测定。该研究将是 与赞助商 David G. I. Kingston 博士合作进行 弗吉尼亚理工学院和州立大学。申请人有 曾任维尔京群岛大学的教员 历史上是美属维尔京群岛的黑人大学，最近他 已被推荐终身任职。申请人曾参与 在他的整个职业生涯中，他对本科少数族裔学生进行了研究 紫外线。拟议的研究培训将为 各种生物测定程序和最先进技术的申请人 用于天然产物的结构测定，进而 将使他能够继续在这个领域进行真正独立的研究计划 UVI 场。