Oklahoma COBRE in Structural Biology

俄克拉荷马州 COBRE 结构生物学

• 批准号: 10197142
• 负责人: ANN H WEST
• 金额: $ 211.29万
• 依托单位: UNIVERSITY OF OKLAHOMA
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-01 至 2023-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10197142
• 关键词: Acute; Area; Award; Bacterial Infections; Biological Sciences; Businesses; Centers of Research Excellence; Chronic; Communities; Core Facility; Crystallization; Data Collection; Development; Disease; Education; Education and Outreach; Educational workshop; Ensure; Equipment; Evaluation; Extramural Activities; Faculty; Funding; Future; Goals; Grant; Health Sciences; Interdisciplinary Study; Malignant Neoplasms; Mentors; Mentorship; Molecular Structure; Neurodegenerative Disorders; Oklahoma; Paper; Peer Review; Phase; Pilot Projects; Postdoctoral Fellow; Preparation; Production; Proteins; Publishing; Research; Research Activity; Research Infrastructure; Research Personnel; Research Project Grants; Research Training; Resource Sharing; Roentgen Rays; Sampling; Stroke; Structure; Students; Training; biomedical resource; biophysical analysis; career; career development; faculty support; human disease; instrumentation; macromolecule; novel therapeutics; posters; programs; protein expression; protein purification; structural biology; symposium; undergraduate student

PROJECT SUMMARY (Overall Component) Structural biology is a multidisciplinary research area that focuses on the important relationship between macromolecular structure and function. The Oklahoma Center of Biomedical Research Excellence (COBRE) in Structural Biology (OCSB) was established in 2012 with Phase I funding. The OCSB supports faculty research projects and three core facilities on the OU-Norman and OU Health Sciences Center campuses for crystallization of macromolecules of biomedical importance, X-ray data collection, protein expression and purification and biophysical analysis. Structural biology lies at the intersection of many different areas of biological sciences and thus has the potential of impacting numerous biomedically important fields. The research projects proposed herein have direct relevance to human diseases and conditions associated with neurodegenerative diseases, cancer, stroke, and both chronic and acute bacterial infections. The overall objective of this Phase II application is to continue to build and nurture a critical mass of researchers in structural biology and support their research programs through shared resources and expertise. The specific aims of this Phase II application are to: 1) Support and augment the research activities and career development of junior and early career investigators through senior mentorship and enhanced research infrastructure with the goal of enhancing their ability to compete successfully for independent external funding; 2) Continue to provide state-of-the-art research core facilities in support of structural biology researchers by expanding the capabilities of these cores and developing a path to self-sustainability; and 3) Continue to promote and enhance the visibility of structural biology in the State of Oklahoma through OCSB-sponsored activities such as annual symposia, workshops, a pilot project program, and undergraduate and graduate research and education programs.

项目摘要（总体组成部分） 结构生物学是一个多学科研究领域，重点关注结构生物学之间的重要关系 大分子结构和功能。俄克拉荷马州生物医学卓越研究中心 (COBRE) 结构生物学博士 (OCSB) 成立于 2012 年，获得一期资金。 OCSB 支持教师 OU-诺曼和 OU 健康科学中心校园的研究项目和三个核心设施 具有生物医学重要性的大分子结晶、X 射线数据收集、蛋白质表达和 纯化和生物物理分析。结构生物学位于许多不同领域的交叉点 生物科学，因此有可能影响许多生物医学重要领域。这 本文提出的研究项目与人类疾病和状况直接相关 神经退行性疾病、癌症、中风以及慢性和急性细菌感染。整体 第二阶段申请的目标是继续培养和培养关键数量的研究人员 结构生物学并通过共享资源和专业知识支持他们的研究项目。具体的 第二阶段申请的目的是： 1) 支持和增强研究活动和职业生涯 通过高级指导和加强研究来培养初级和早期职业研究人员 基础设施，旨在增强其成功竞争独立外部资金的能力； 2）继续提供最先进的研究核心设施，以支持结构生物学研究人员 扩大这些核心的能力并开辟一条自我可持续发展的道路； 3) 继续 通过 OCSB 赞助，促进和提高结构生物学在俄克拉荷马州的知名度 年度研讨会、讲习班、试点项目计划以及本科生和研究生等活动 研究和教育计划。

项目成果

Organometallic myoglobins: Formation of Fe-carbon bonds and distal pocket effects on aryl ligand conformations.

有机金属肌红蛋白：铁-碳键的形成和对芳基配体构象的远端口袋效应。

• 发表时间: 2016-11
• 影响因子: 3.9
• 作者: Wang, Bing;Thomas, Leonard M;Richter
• 通讯作者: Richter

Structure-function analyses reveal a triple β-turn receptor-bound conformation of adrenomedullin 2/intermedin and enable peptide antagonist design.

结构功能分析揭示了肾上腺髓质素 2/intermedin 的三重 β 转角受体结合构象，并使肽拮抗剂设计成为可能。

• 发表时间: 2018
• 作者: Roehrkasse, Amanda M;Booe, Jason M;Lee, Sang;Warner, Margaret L;Pioszak, Augen A
• 通讯作者: Pioszak, Augen A

Protein features for assembly of the RNA editing helicase 2 subcomplex (REH2C) in Trypanosome holo-editosomes.

锥虫全编辑体中 RNA 编辑解旋酶 2 亚复合物 (REH2C) 组装的蛋白质特征。

• 发表时间: 2019
• 影响因子: 3.7
• 作者: Kumar, Vikas;Doharey, Pawan K;Gulati, Shelly;Meehan, Joshua;Martinez, Mary G;Hughes, Karrisa;Mooers, Blaine H M;Cruz
• 通讯作者: Cruz

Acceptor substrate determines donor specificity of an aromatic prenyltransferase: expanding the biocatalytic potential of NphB.

受体底物决定芳香族异戊二烯基转移酶的供体特异性：扩大 NphB 的生物催化潜力。

• 发表时间: 2020-05
• 影响因子: 5
• 作者: Johnson, Bryce P;Scull, Erin M;Dimas, Dustin A;Bavineni, Tejaswi;Bandari, Chandrasekhar;Batchev, Andrea L;Gardner, Eric D;Nimmo, Susan L;Singh, Shanteri
• 通讯作者: Singh, Shanteri

PEGylation of Polyethylenimine Lowers Acute Toxicity while Retaining Anti-Biofilm and β-Lactam Potentiation Properties against Antibiotic-Resistant Pathogens.

聚乙烯亚胺的聚乙二醇化降低了急性毒性，同时保留了针对抗生素耐药病原体的抗生物膜和β-内酰胺增强特性。

• 发表时间: 2020-10-13
• 影响因子: 4.1
• 作者: Lam, Anh K;Moen, Erika L;Pusavat, Jennifer;Wouters, Cassandra L;Panlilio, Hannah;Ferrell, Maya J;Houck, Matthew B;Glatzhofer, Daniel T;Rice, Charles V
• 通讯作者: Rice, Charles V