ShEEP Request for Zeiss Modular Laser Scanning Microscope LSM 980

ShEEP 请求蔡司模块化激光扫描显微镜 LSM 980

• 批准号: 10175791
• 负责人: Gregory A. Elder
• 金额: --
• 依托单位: JAMES J PETERS VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-10-01 至 2021-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10175791
• 关键词: 3-Dimensional; Alzheimer' s Disease; Area; Core Facility; Detection; Disease; Environment; Equipment; Goals; Health; Image; Imaging Techniques; Imaging technology; Lasers; Malignant Neoplasms; Medical center; Mental disorders; Microscope; Microscopy; Performance; Program Development; Research; Research Personnel; Research Project Grants; Resolution; Scanning; Sheep; Spinal cord injury; System; Training; Traumatic Brain Injury; Veterans; confocal imaging; in vivo imaging; multiphoton imaging; multiphoton microscopy; operation; optical imaging; research and development

The Research & Development Program at the James J. Peters VA Medical Center (JJPVAMC) has for many years supported a variety of core equipment items that are available to VA investigators and their collaborators. Among the most utilized core equipment items are a set of microscopes that function as part of a Microscopy Core Facility. Central to the operations of this Microscopy Core is a Zeiss LSM 700 microscope that was purchased 7 years. This microscope has been a workhorse that has provided JJPVAMC researches with high-resolution laser scanning confocal imaging for many years. However, with advances in imaging technology, this microscope is now lacks the sensitivity and important functionalities that are essential for high performance confocal/multiphoton imaging and in vivo imaging applications. The goal of this application is to replace an outdated Zeiss LSM700 microscope with an optimally configured and fully functional confocal/multiphoton microscope system that will be be made available to JJPVAMC investigators. We propose to replace the LSM 700 system with a Zeiss LSM 980 confocal/multiphoton inverted laser-scanning microscope with Airyscan detection for 3D super-resolution imaging. The LSM 980 confocal/multiphoton system will be placed in a multiuser environment. Technical support as well user training will be provided to support all VA research projects that might benefit from high-end imaging. Acquisition of an LSM 980 confocal/multiphoton system will provide a cutting-edge system for high resolution laser scanning confocal/multiphoton microscopy and related high-end optical imaging techniques.

James J. Peters VA 医疗中心 (JJPVAMC) 的研发计划 多年来支持各种核心设备项目，供 VA 调查人员及其人员使用 合作者。最常用的核心设备之一是一套显微镜，其功能是作为 显微镜核心设施。该显微镜核心的操作核心是蔡司 LSM 700 显微镜 购买了7年。该显微镜一直是 JJPVAMC 研究的主力 多年来一直致力于高分辨率激光扫描共焦成像。然而随着成像技术的进步 技术，该显微镜现在缺乏高灵敏度和重要功能所必需的 性能共焦/多光子成像和体内成像应用。该应用程序的目标是 用优化配置且功能齐全的显微镜替换过时的蔡司 LSM700 显微镜 共焦/多光子显微镜系统将提供给 JJPVAMC 研究人员。我们 建议将 LSM 700 系统替换为 Zeiss LSM 980 共焦/多光子倒置激光扫描 具有 Airyscan 检测功能的显微镜，可进行 3D 超分辨率成像。 LSM 980 共焦/多光子 系统将被置于多用户环境中。将提供技术支持以及用户培训 支持所有可能受益于高端成像的 VA 研究项目。购买 LSM 980 共焦/多光子系统将为高分辨率激光扫描提供尖端系统 共焦/多光子显微镜和相关的高端光学成像技术。