Upgrade the 14T Ultrahigh Field Horizontal MR Scanner for Rodent and ex-vivo Imaging

升级 14T 超高场水平 MR 扫描仪，用于啮齿动物和离体成像

基本信息

批准号：
10175835
负责人：
BRUCE R ROSEN
金额：
$ 60万
依托单位：
MASSACHUSETTS GENERAL HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-04-15 至 2024-04-14
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10175835
关键词：
Alzheimer&apos s Disease Anatomy Animal Disease Models Animal Model Animals Biosensor Brain Brain Diseases Brain Mapping Calcium Coma Dementia Diffusion Magnetic Resonance Imaging Electronics Functional Magnetic Resonance Imaging Funding General Hospitals Glutamates Goals Heart Arrest Hepatic Fibrogenesis Hepatitis Human Image Magnetic Resonance Imaging Massachusetts Mediating Methodology Methods Migraine Multimodal Imaging Organ Parkinson Disease Performance Research Personnel Research Project Grants Resolution Resources Rodent Signal Transduction Software Design System Techniques Tissue imaging Translations Traumatic Brain Injury United States National Institutes of Health animal imaging base brain tissue cerebrovascular ex vivo imaging functional MRI scan imaging platform improved instrument multidisciplinary multimodality neuroimaging neurovascular next generation novel optical fiber optogenetics pre-clinical technological innovation translational study

项目摘要

The goal of this proposal is to purchase a Bruker AV-Neo console to replace the out-of-date Siemens console of the 14T horizontal MR scanner (13cm bore), which has been dedicated to supporting the high-resolution anatomical and functional dynamic brain mapping of animals and ex-vivo human brain tissue imaging. The Martinos Center at the Massachusetts General Hospital has been at the forefront of developing advanced functional mapping methods, e.g., functional MRI, and implementing the cutting-edge MRI methods to bridge the basic and translational studies. To pursue the next-generation cutting-edge imaging methodology and prepare for the higher field MRI translational studies, there is an urgent need to improve our 14T MRI preclinical platform for high-resolution animal and ex vivo imaging. In particular, the proposed 14T MR console upgrade will boost the translational potentials of the novel methodology, e.g. the single- vessel fMRI and line-scanning fMRI, to bridge cellular and microvascular anatomy and functional dynamics from animal to human brains. Also, this proposal will support over 20 research projects funded by NIH, presenting critical translational efforts on the mechanistic studies of brain disorders including Alzheimer’s Diseases (AD), Parkinson’s Disease, cerebrovascular dementia, migraine, traumatic brain injury, and cardiac arrest (AC)-induced coma, as well as for the internal organ imaging of animal models with hepatitis and hepatic fibrogenesis. The highly synergistic and collaborative research projects outlined in our proposal can be summarized in three main themes: 1) Neurovascular dynamic signaling, 2) Cutting-edge neuroimaging methodology, 3) Multimodal mechanistic signatures of animal disease models. We have established the 14T- based multi-modal imaging platform to combine the high-resolution anatomical and functional MRI imaging with the emerging neuro-techniques, e.g. optogenetics, optical fiber-mediated biosensor recording of Calcium, Glutamate, etc, promoting novel mechanistic understanding of the complexity of brain function. The Bruker AV-Neo system will provide key technological innovations to improve the performance of the novel brain mapping methods, e.g. the single-vessel fMRI, line- scanning fMRI, RF slab-specific diffusion-weighted MRI, based on the state-of-the-art electronics and software design. Therefore, the proposed instrument upgrade would not only accelerate the progress of the listed projects but also facilitate the translation of cutting-edge MR methodologies as a truly multidisciplinary, regional resource for PHS funded investigators.

该提案的目标是购买布鲁克 AV-Neo 控制台来替换过时的西门子 14T 卧式 MR 扫描仪（13cm 孔径）控制台，专门用于支持动物的高分辨率解剖和功能动态大脑绘图马萨诸塞州总医院马蒂诺斯中心的离体人类脑组织成像。一直处于先进功能映射方法开发的前沿，例如功能 MRI，并实施最先进的 MRI 方法来连接基础和转化研究。追求下一代尖端成像方法并为高场MRI转化研究，迫切需要改进我们的14T MRI临床前用于高分辨率动物和离体成像的平台，特别是所提出的 14T MR。控制台升级将增强新方法的转化潜力，例如血管功能磁共振成像和线扫描功能磁共振成像，连接细胞和微血管的解剖结构和功能此外，该提案还将支持 20 多个研究项目。由 NIH 资助，提出脑机制研究的关键转化工作疾病包括阿尔茨海默病（AD）、帕金森病、脑血管痴呆、偏头痛、创伤性脑损伤和心脏骤停 (AC) 引起的昏迷，以及内部肝炎和肝纤维化动物模型的器官成像具有高度协同作用。我们提案中概述的合作研究项目可以概括为三个主要方面主题：1) 神经血管动态信号传导，2) 尖端神经影像方法，3) 我们建立了动物疾病模型的多模态机制特征。基于多模态成像平台，结合高分辨率解剖和功能 MRI 使用新兴神经技术进行成像，例如光遗传学、光纤介导的生物传感器记录钙、谷氨酸等，促进对钙、谷氨酸等的新机制理解布鲁克 AV-Neo 系统将提供关键的技术创新。提高新型脑图方法的性能，例如单血管功能磁共振成像、线扫描功能磁共振成像 (fMRI)、射频板特定扩散加权磁共振成像 (MRI)，基于最先进的电子设备因此，拟议的仪器升级不仅会加速所列项目的进展，同时也促进尖端MR方法的转化作为 PHS 资助的研究人员真正的多学科、区域资源。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Characterizing brain stage-dependent pupil dynamics based on lateral hypothalamic activity.

基于侧下丘脑活动表征大脑阶段依赖的瞳孔动态。

DOI：
发表时间：
2023-10-14
期刊：
影响因子：
0
作者：
Takahashi, Kengo;Sobczak, Filip;Pais;Yu, Xin
通讯作者：
Yu, Xin

Novel inductively coupled ear-bars (ICEs) to enhance restored fMRI signal from susceptibility compensation in rats.

新型电感耦合耳棒 (ICE) 可增强大鼠磁敏补偿恢复的功能磁共振成像信号。

DOI：
发表时间：
2024-01-14
期刊：
影响因子：
0
作者：
Chen, Yi;Fernandez, Zachary;Scheel, Norman;Gifani, Mahsa;Zhu, David C;Counts, Scott E;Dorrance, Anne M;Razansky, Daniel;Yu, Xin;Qian, Wei;Qian, Chunqi
通讯作者：
Qian, Chunqi

Laminar-specific functional connectivity mapping with multi-slice line-scanning fMRI.

使用多切片线扫描功能磁共振成像进行层流特异性功能连接映射。

DOI：
发表时间：
2022-10-08
期刊：
影响因子：
0
作者：
Choi, Sangcheon;Zeng, Hang;Chen, Yi;Sobczak, Filip;Qian, Chunqi;Yu, Xin
通讯作者：
Yu, Xin

Cerebrovascular Gi Proteins Protect Against Brain Hypoperfusion and Collateral Failure in Cerebral Ischemia.

脑血管 Gi 蛋白可预防脑缺血时的脑灌注不足和附带衰竭。

DOI：
发表时间：
2023-04
期刊：
影响因子：
3.1
作者：
Castaneda;Beer;Leiss, Veronika;Napieczyńska, Hanna;Vuozzo, Marta;Schmid, Andreas M;Zeng, Hang;He, Yi;Kohlhofer, Ursula;Gonzalez;Quintanilla;Hempel, Johann;Gollasch, Maik;Y
通讯作者：
Y

Challenges and Perspectives of Mapping Locus Coeruleus Activity in the Rodent with High-Resolution fMRI.

用高分辨率功能磁共振成像绘制啮齿类动物蓝斑活动的挑战和前景。