Biopolymers & Proteomics

生物聚合物

• 批准号: 10171796
• 负责人: MICHAEL B YAFFE
• 金额: $ 35.86万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-17 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10171796
• 关键词: Binding; Biocompatible Materials; Biological; Biopolymers; Budgets; Cancer Center; Cancer Center Support Grant; Cells; Complex; Consultations; Core Grant; Data Analyses; Detection; Development; Disease; Experimental Designs; Fractionation; Funding; High Pressure Liquid Chromatography; Hybrids; Individual; Informatics; Information Centers; Institutes; Mass Spectrum Analysis; Membrane; Methods; Mission; Modernization; Modification; Monitor; Organism; Peptide Synthesis; Peptides; Pharmaceutical Preparations; Post-Translational Protein Processing; Proteins; Proteomics; Research; Research Personnel; Resource Sharing; Resources; Sampling; Sensitivity and Specificity; Services; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Speed; Technical Expertise; Techniques; Technology; Tissues; Training and Education; Update; Vendor; anticancer research; base; equipment acquisition; improved; instrument; instrumentation; interest; member; metabolomics; operation; programs; relative cost; response; small molecule; success; training opportunity

Biopolymers & Proteomics Core: Project Summary/Abstract The ability to sensitively and quantitatively analyze proteins, peptides, small molecules and metabolites is critical to understanding differences between normal and disease states in cells, tissues and organisms. Such analyses typically require sophisticated technical expertise and state-of-the-art instrumentation. The Biopolymers & Proteomics Core (B&P Core) provides Koch Institute researchers with a diverse range of services, with an emphasis on protein/peptide, proteomic and mass spectrometry services, that enable both synthesis and analysis of biological materials. These are technologies that individual researchers usually do not learn, but are essential for modern cancer research, and as such the Core provides access to these techniques. In the current funding period, the B&P Core added new capabilities and modified existing services, including: acquisition of two new hybrid quadrupole-orbitrap MS instruments with funds from Institutional sources to expand proteomics analysis capacity and improve sensitivity; upgraded HPLC with funds from Institutional sources to enable improved fractionation of complex samples and added independent HPLC use; and expanded expertise in HPLC analysis and purification (e.g. small molecule-drug conjugates). Center Member Core use has been strong: 84% of Center Members use the Core, account for 74% of Core service use, and include investigators from all three Research Programs. In the upcoming period, the B&P Core will continue to offer a wide range of state-of-the-art services to support Center Member research programs, and will evaluate emerging capabilities in the context of Center Member needs and interests. A number of new initiatives are planned, including: expansion and enhancement of proteomics capabilities in global and quantitative proteomic analysis, detection of post-translational modifications and identification of binding partner profiling; and improved investigator support for mass spectrometry-related informatics and complex proteomic data analyses. The B&P Core is also committed to maintaining instrumentation at the leading edge, and will continue to closely monitor technological improvements, particularly with regard to LC-MS instrument sensitivity, specificity, operation, and speed. The Core has taken advantage of leasing and vendor consignment programs to provide more frequent instrumentation updates, allowing the Core to pilot new advances and technologies at lower relative cost. In the upcoming funding period, we will conduct a hands-on assessment of Center Member needs with respect to metabolomics capabilities. Funding for any new equipment acquisitions will be solely from Institutional sources. This Shared Resource is essential to the success of the Koch Institute mission and provides exceptional value to the CCSG. The requested CCSG budget for Year 49 is increased by 18.5% over the Core CCSG budget for the current period (Year 48), reflecting increased support for proteomics expertise.

生物聚合物和蛋白质组学核心：项目摘要/摘要 灵敏定量分析蛋白质、肽、小分子和代谢物的能力至关重要 了解细胞、组织和生物体的正常状态和疾病状态之间的差异。这样的分析 通常需要复杂的技术专业知识和最先进的仪器。生物聚合物 & 蛋白质组学核心（B&P Core）为科赫研究所研究人员提供多样化的服务， 强调蛋白质/肽、蛋白质组学和质谱服务，使合成和分析成为可能 生物材料分析。这些是个人研究人员通常不会学习的技术，但 对于现代癌症研究至关重要，因此核心提供了使用这些技术的途径。 在当前资助期内，B&P Core 添加了新功能并修改了现有服务，包括： 利用机构来源的资金购买两台新型混合四极轨道阱 MS 仪器以进行扩展 蛋白质组学分析能力和灵敏度提高；利用机构来源的资金升级 HPLC 改进复杂样品的分馏并增加独立的 HPLC 使用；和扩展的专业知识 HPLC 分析和纯化（例如小分子药物缀合物）。中心会员核心使用已 强：84% 的中心成员使用核心，占核心服务使用的 74%，其中包括调查员 来自所有三个研究计划。 在接下来的一段时间内，B&P Core 将继续提供广泛的最先进的服务来支持 中心成员研究计划，并将评估中心成员背景下的新兴能力 需求和兴趣。计划采取一系列新举措，包括： 扩大和加强 全局和定量蛋白质组分析、翻译后修饰检测的蛋白质组能力 以及结合伙伴分析的识别；并改进了研究者对质谱相关的支持 信息学和复杂的蛋白质组数据分析。 B&P Core 还致力于维护 仪器仪表处于领先地位，并将继续密切关注技术进步，特别是 关于 LC-MS 仪器的灵敏度、特异性、操作和速度。核心利用了 租赁和供应商寄售计划可提供更频繁的仪器更新，从而使核心 以较低的相对成本试点新的进步和技术。在接下来的资助期内，我们将进行 对中心成员在代谢组学能力方面的需求进行实际评估。为任何新项目提供资金 设备采购将完全来自机构来源。此共享资源对于成功至关重要 科赫研究所的使命，并为 CCSG 提供了非凡的价值。请求的 CCSG 年度预算 49 比本期（第 48 年）核心 CCSG 预算增加 18.5%，反映出增加的 支持蛋白质组学专业知识。