High-definition, wide field of view corneal imaging

高清、宽视场角膜成像

基本信息

批准号：
10172909
负责人：
Cristina Canavesi
金额：
$ 74.2万
依托单位：
LIGHTOPTECH CORPORATION
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-01 至 2023-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10172909
关键词：
3-Dimensional Address Area Assessment tool Blindness Cell Count Cell Density Cell Survival Cell Viability Process Cellular Morphology Characteristics Clinic Confocal Microscopy Cornea Corneal Diseases Disease Endothelial Cells Evaluation Eye Eye Banks Goals Gold Grant Health Human Image Imaging Device Imaging Techniques Industry Infection Innovation Corps International Lead Legal patent Machine Learning Measurement Measures Methods Microscope Microscopy Monitor Morphology Operative Surgical Procedures Ophthalmology Optics Organ Transplantation Patient imaging Phase Positioning Attribute Process Regenerative capacity Research Resolution Rights Sampling Sampling Errors Small Business Innovation Research Grant Standardization Structure Technology Thick Thinness Time Tissue Donors Tissue Transplantation Tissues Training Transplantation United States National Institutes of Health Universities Variant Visual Visual impairment Visual system structure Visualization software base clinical development clinical imaging commercial application density image processing improved in vivo in vivo regeneration innovation instrument instrumentation microscopic imaging multidisciplinary neurosensory novel phase 2 study programs prototype screening three-dimensional visualization tool trend

项目摘要

The cornea is the primary focusing structure of our visual system. Infections and diseases in the tissue can impair vision and lead to blindness, even in eyes with intact neurosensory function. Corneal disease is one of the leading causes of visual deficiency and blindness in the world. Tissue evaluation is an important step for assessing the health of the donor cornea and its appropriateness for different types of placement, yet this process suffers from high subjectivity. High-definition corneal imaging is needed to assist in selection of the most appropriate tissue for transplant. Progress on this front would greatly serve public need, as the cornea is the most commonly transplanted tissue worldwide, with nearly 185,000 transplants annually. Thus, a more sensitive and quantitative method for objective evaluation of tissue at eye banks is needed. We have developed a 3D high-definition imaging instrument based on Gabor-Domain Optical Coherence Microscopy (GDOCM). Our SBIR Phase I research successfully accomplished all Aims and demonstrated the feasibility of quantitative assessment of corneal tissue over a large field of view with GDOCM. Our Phase I results demonstrated that GDOCM has the following key advantages over existing corneal imaging techniques, which include specular and confocal microscopy: 1) improved accuracy of tissue qualification with 4-10x increase in field of view that reduces sampling error – this will provides a truer assessment of the overall tissue characteristics; 2) ability to simultaneously measure corneal thickness, quantify endothelial cell density, and identify morphological variations due to corneal disease – this will lead to complete corneal evaluation in a single instrument; 3) leveraging machine learning innovations to minimize variability induced by users – this will result in a more objective evaluation; 4) enhanced 3D cellular-level imaging of thin translucent endothelial cells – this will enable a detailed understanding of cell viability. The results of the proposed Phase studies II will demonstrate that GDOCM can provide high-definition, 3D visualization of corneal structures with immediate commercial application for qualification of donor tissue in eye banks, and with a path to in vivo clinical imaging of patients with corneal disease.

角膜是我们视觉系统的主要聚焦结构，组织中的感染和疾病可能会发生。损害视力并导致失明，即使眼睛神经感觉功能完好，角膜疾病也是其中之一。组织评估是世界上视力缺陷和失明的主要原因。评估供体角膜的健康状况及其对不同类型放置的适当性，但这该过程具有高度主观性，需要高清角膜成像来协助选择。最适合移植的组织。这方面的进展将极大地满足公众的需求，就像角膜一样。全世界最常移植的组织，每年有近 185,000 例移植手术。需要灵敏且定量的方法来客观评估眼库组织。我们开发了基于Gabor域光学相干的3D高清成像仪器显微镜 (GDOCM)。我们的 SBIR 第一阶段研究成功实现了所有目标并证明了使用 GDOCM 对大视野角膜组织进行定量评估的可行性。与现有的角膜成像技术相比，GDOCM 具有以下主要优势：包括镜面和共焦显微镜：1) 组织鉴定的准确性提高了 4-10 倍减少采样误差的视野——这将为整个组织提供更真实的评估特征；2) 同时测量角膜厚度、量化内皮细胞密度的能力，以及识别由于角膜疾病引起的形态变化——这将导致一次完整的角膜评估 3）利用机器学习创新来最大限度地减少用户引起的变化——这将导致更客观的评估；4）增强薄半透明内皮细胞的 3D 细胞水平成像 - 这将有助于详细了解细胞活力。拟议的第二阶段研究的结果将证明 GDOCM 可以提供高清、3D 角膜结构的可视化可立即商业应用，用于眼部供体组织的鉴定库，并为角膜疾病患者提供了体内临床成像的途径。