NONINVASIVE ASSESSMENT OF EMBRYO VIABILITY IN VITRO

体外胚胎活力的无创评估

• 批准号: 2203886
• 负责人: GUNTER A HOFMANN
• 金额: $ 7.88万
• 依托单位: GENETRONICS, INC.
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-02-01 至 1994-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2203886
• 关键词: biomedical equipment development; cow; early embryonic stage; embryo /fetus culture; embryo /fetus monitoring; embryo /fetus transplantation; in vitro fertilization; laboratory mouse; noninvasive diagnosis; oxygen consumption; vertebrate embryology

Embryo transfer (ET) procedures have gained widespread usage in modern human and animal programs of assisted reproduction. However, to optimize ET success rates, an objective test is needed to accurately determine the viability of individual preimplantation embryos: l) following embryo collection, 2) after conducting various procedures of assisted reproduction (IVM/IVF/cryopreservation), and 3) prior to transfer to a synchronized recipient. The short-term goal of the Phase I SBIR proposal is to design, produce and test a portable, high-resolution, prototype system that is capable of performing a rapid, non-invasive assessment of the viability of a single mammalian embryo in vitro. Our working hypothesis states that embryo oxygen consumption (QO2 = respiration) is a direct measure of that embryo's potential to survive in vivo following ET. The future potential of this research is to commercialize a novel, high- resolution, micro-respiration apparatus that is capable of predicting the "viability" of an individual oocyte/embryo. By directly testing the sensitivity, resolution and user-friendly nature of this technology, results of this Phase I project will define the applicability of the technology, and will provide the necessary technical basis to support a Phase II application to further modify and field-test the "embryo viability system" for commercialization.

胚胎转移（ET）程序已在现代中广泛使用 辅助生殖的人类和动物计划。 但是，要优化 ET成功率，需要一项客观测试，以准确确定 单个植入前胚胎的生存能力：l）胚胎后 收集，2）执行了各种辅助程序之后 复制（IVM/IVF/冷冻保存），3）转移到A之前 同步收件人。 I阶段SBIR提案的短期目标 是设计，生产和测试便携式，高分辨率，原型 能够进行快速无创评估的系统 单个哺乳动物胚胎在体外的生存能力。 我们的工作 假设指出，胚胎消耗（QO2 =呼吸）是 直接测量该胚胎在ET之后在体内生存的潜力。 这项研究的未来潜力是将新颖的高级商业化 分辨率，能够预测的微吸气设备 单个卵母细胞/胚胎的“生存能力”。 通过直接测试 该技术的敏感性，分辨率和用户友好性， 该阶段I项目的结果将定义 技术，并将提供必要的技术依据来支持 II阶段应用以进一步修改和现场测试“胚胎 可活力系统“用于商业化。