Investigation of halophilic microorganisms in salt lakes and development of novel halogenating and dehalogenating enzymes

盐湖中嗜盐微生物的研究及新型卤化和脱卤酶的开发

基本信息

批准号：
10041167
负责人：
ESAKI Nobuyoshi
金额：
$ 2.82万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10041167/
关键词：
Salt lake halogen metabolism halogenating enzyme dehalogenating enzyme

项目摘要

We collected halophilic microorganisms from salt lakes containing various kinds of salts of various concentrations in order to obtain novel enzymes involved in halogen metabolism, catalyzing halogenation and dehalogenation. Taking advice from Professor Mike Dyall-Smith at University of Melbourne in Australia, we collected water samples from several salt lakes in Rottnest Island in West Australia, from which various halophilic microorganisms were isolated. We also isolated halophiles from salt lakes, such as Mono Lake, in California, USA. We carried out screening using media containing on of the following halogenated compounds : 4, 6-dichlororesorcinol, 1, 2-dichloroethane, fluoroacetate, 4-chlororesorcinol, 2-chloropropionate, 2-chloropropionamide, glycerol-α-monochlorohydrin, and chloroacetate. We observed that microorganisms grew in the media containing 4, 6-dichlororesorcinol, 1, 2-dichloroethane, or fluoroacetate. Cocci were found in the culture obtained with 4, 6-dichlororesorcinol, suggesting that this microorganism produces an enzyme degrading 4, 6-dichlororesorcinol or has another mechanism that makes the cell resistant to this compound. We also collaborated with Professor Jiang at Institute of Microbiology, Yunnan University, China, and isolated microorganisms assimilating 3-chloro-1-propanol (one bacterial strain, two actinomycetous strains, and three fungous strains). Bacterial strains that can assimilate chloropropionate and glycerol-α-monochlorohydrin were also obtained.

我们从含有不同浓度的各种盐的盐湖中收集嗜盐微生物，以获得参与卤素代谢、催化卤化和脱卤的新型酶，并在澳大利亚墨尔本大学Mike Dyall-Smith教授的建议下，采集了水样。我们还从西澳大利亚罗特内斯特岛的几个盐湖中分离出了各种嗜盐微生物，例如莫诺湖、在美国加利福尼亚州，我们使用含有以下卤代化合物的介质进行了筛选：4, 6-二氯间苯二酚、1, 2-二氯乙烷、氟乙酸酯、4-氯间苯二酚、2-氯丙酸酯、2-氯丙酰胺、甘油-α-单氯丙醇。和氯乙酸盐，我们观察到微生物在含有 4、在用 4, 6-二氯间苯二酚获得的培养物中发现了 6-二氯间苯二酚、1, 2-二氯乙烷或氟乙酸酯，这表明该微生物产生了一种降解 4, 6-二氯间苯二酚的酶，或者具有使细胞对 4, 6-二氯间苯二酚产生抗性的另一种机制。我们还与中国云南大学微生物研究所姜教授合作，分离出了该化合物。还获得了可同化3-氯-1-丙醇的微生物（1株细菌、2株放线菌菌株、3株真菌菌株）。