LOCAL IMMUNE RESPONSE IN CHLAMYDIAL SALPINGITIS

衣原体输卵管炎的局部免疫反应

• 批准号: 2061700
• 负责人: DOROTHY L PATTON
• 金额: $ 20.09万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-01 至 1997-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2061700
• 关键词: B lymphocyte; Chlamydia trachomatis; Macaca nemestrina; T lymphocyte; active immunization; antibacterial antibody; antibody formation; bacterial antigens; cell sorting; cellular pathology; cytokine; delayed hypersensitivity; disease /disorder model; fallopian tubes; fertility; gene expression; histopathology; immunocytochemistry; immunopathology; messenger RNA; molecular pathology; northern blottings; pathologic process; salpingitis; western blottings

The Objective of the proposed study is to elucidate the molecular and cellular immunological responses at the local site of infection during the course of acute and chronic chlamydia infection in the salpinx primate pocket model. The experimental approach will generate information about immune cell subsets with cytokine assays, immunohistochemical data, and mRNA expression from the tissues representing different stages of chlamydia infection to ascertain their role(s) in scarring and fibrosis associated with tubal infertility. The specific aims are: i) Phenotype the principal components of the local inflammatory infiltrate including subsets of T and B cells; 2) Identify cytokine expression within acute and chronic lesions; 3) characterize the function and specificity of lesion lymphocytes (T and B cell subpopulations) and peripheral blood, and evaluate their roles in the local inflammatory process; and 4) Isolation of T cell lines and clones that are specific for Chlamydia trachomatis. These studies will contribute not only to the understanding of the molecular and cellular basis of the inflammatory response to C. trachomatis salpingitis, but also to the development of effective means of treatment and prevention of infertility and ectopic pregnancy.

拟议研究的目的是阐明分子和 在局部感染部位的细胞免疫反应期间 Salpinx Primate中急性和慢性衣原体感染的过程 口袋模型。 实验方法将生成有关的信息 具有细胞因子测定，免疫组织化学数据和 来自代表不同阶段的组织的mRNA表达 衣原体感染以确定其在疤痕和纤维化中的作用 与输卵管不育症有关。具体目的是：i）表型 局部炎症性浸润的主要成分，包括 T和B细胞的子集； 2）确定急性和 慢性病变； 3）表征病变的功能和特异性 淋巴细胞（T和B细胞亚群）和外周血，以及 评估其在局部炎症过程中的作用； 4）隔离 T细胞系和克隆的特定于沙眼衣原体。 这些研究不仅将有助于理解 炎症反应的分子和细胞基础。 气管造口炎，但也要开发有效手段 治疗和预防不育和异位妊娠。